Evidence for the existence of an NRAMP1 gene in the horse

Based on primers designed according to conserved sequences of the NRAMP1 gene, horse genomic DNA was used for amplification, cloning, and sequencing of the putative horse orthologue. Sequence alignment revealed high level of homologies with other mammalian species ranging from 93.1 % (sheep) to 85.1 % (mouse). The horse sequences were identified as exons 3, 4, 5, and 6. The amino acid position corresponding to the site 169 of the gly to asp substitution in mice is occupied by glycine in the hone. Differences between Old Kladruber and Akhal-Teke hones in allelic frequencies of two intronic RFLP markets of the horse NRAMP1 gene were found.