Isolation and characterization of a new trypsin inhibitor from Crotalaria paulina seeds

被引：16
作者：
Pando, LA
Di Ciero, L
Novello, JC
Oliveira, B
Weder, JKP
Marangoni, S [1 ]
机构：
[1] Univ Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazil
[2] Univ Sao Paulo, ESALQ, Dept Fitopatol, Piracicaba, SP, Brazil
[3] Tech Univ Munich, Inst Lebensmittelchem, D-85748 Garching, Germany
来源：
IUBMB LIFE | 1999年 / 48卷 / 05期
关键词：
Crotalaria paulina; trypsin inhibitor;
D O I：
10.1080/152165499306621
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new trypsin inhibitor (CPTI) has been isolated from Crotalaria paulina seeds. Purification of the inhibitor was carried out by gel filtration, ion-exchange chromatography, and subsequent reversed-phase HPLC. The presence of a single polypeptide chain, with a molecular mass of 20 kDa and isoelectric point 4.0, was detected. The trypsin inhibitor had a K-i value of 4.5 x 10(-8) M and was capable of acting on human, bovine, and porcine trypsin and weakly on bovine chymotrypsin, Amino acid analysis showed that CPTI has a high content of aspartate, glutamate, leucine, serine, and glycine, having 177 amino acid residues in its composition. These data suggest that the protein belongs to the Kunitz-type trypsin inhibitors.
引用
收藏
页码：519 / 523
页数：5
相关论文
共 50 条
  • [21] Isolation of a trypsin inhibitor from Echinodorus paniculatus seeds by affinity chromatography on immobilized Cratylia mollis isolectins
    Paiva, PMG
    Souza, AF
    Oliva, MLV
    Kennedy, JF
    Cavalcanti, MSM
    Coelho, LCBB
    Sampaio, CAM
    BIORESOURCE TECHNOLOGY, 2003, 88 (01) : 75 - 79
  • [22] Partial purification and characterization of a trypsin inhibitor isolated from Adenanthera pavonina L. seeds
    de Souza, D. D.
    Brandao-Costa, R. M. P.
    Albuquerque, W. W. C.
    Porto, A. L. F.
    SOUTH AFRICAN JOURNAL OF BOTANY, 2016, 104 : 30 - 34
  • [23] Purification and characterization of trypsin inhibitor from seeds of faba bean (Vicia faba L.)
    Gupta, P
    Dhawan, K
    Malhotra, SP
    Singh, R
    ACTA PHYSIOLOGIAE PLANTARUM, 2000, 22 (04) : 433 - 438
  • [24] Trypsin Inhibitor from Poecilanthe parviflora Seeds: Purification, Characterization, and Activity Against Pest Proteases
    Viviane Alves Garcia
    Maria das Graças Machado Freire
    José Camillo Novello
    Sérgio Marangoni
    Maria Lígia Rodrigues Macedo
    The Protein Journal, 2004, 23 : 343 - 350
  • [25] Trypsin inhibitor from Poecilanthe parviflora seeds:: Purification, characterization, and activity against pest proteases
    Garcia, VA
    Freire, MDM
    Novello, JC
    Marangoni, S
    Macedo, MLR
    PROTEIN JOURNAL, 2004, 23 (05) : 343 - 350
  • [26] Purification and characterization of trypsin inhibitor from seeds of faba bean (Vicia faba L.)
    Parul Gupta
    Kamal Dhawan
    Sarla P Malhotra
    Randhir Singh
    Acta Physiologiae Plantarum, 2000, 22 : 433 - 438
  • [27] Trypsin inhibitor from Dimorphandra mollis seeds:: purification and properties
    Macedo, MLR
    de Matos, DGG
    Machado, OLT
    Marangoni, S
    Novello, JC
    PHYTOCHEMISTRY, 2000, 54 (06) : 553 - 558
  • [28] PURIFICATION OF TRYPSIN CHYMOTRYPSIN INHIBITOR FROM JACK FRUIT SEEDS
    ANNAPURNA, SS
    PRASAD, DS
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1991, 54 (03) : 399 - 411
  • [29] Three-phase partitioning of trypsin inhibitor from legume seeds
    Wati, Richa Kusuma
    Theppakorn, Theerapong
    Benjakul, Soottawat
    Rawdkuen, Saroat
    PROCESS BIOCHEMISTRY, 2009, 44 (12) : 1307 - 1314
  • [30] Isolation and Characterization of a Trypsin Inhibitor and a Lectin from Glycine max cv. Large Black Soybean
    Xiu Juan Ye
    Tzi BunNg
    FOOD SCIENCE AND BIOTECHNOLOGY, 2009, 18 (05) : 1173 - 1179
12345