Thread-based isoelectric focusing coupled with desorption electrospray ionization mass spectrometry

被引：16

作者：

Chen, Liang ^{[1
,2
]}

Cabot, Joan M. ^{[1
,2
]}

Sanz Rodriguez, Estrella ^{[2
]}

Ghiasvand, Alireza ^{[2
,3
]}

Innis, Peter C. ^{[4
]}

Paull, Brett ^{[1
,2
]}

机构：

[1] Univ Tasmania, Sch Nat Sci, ARC Ctr Excellence Electromat Sci ACES, Hobart, Tas 7001, Australia

[2] Univ Tasmania, Sch Nat Sci, Australian Ctr Res Separat Sci ACROSS, Hobart, Tas 7001, Australia

[3] Lorestan Univ, Dept Chem, Khorramabad, Iran

[4] Univ Wollongong, ARC Ctr Excellence Electromat Sci ACES, AIIM Facil, Innovat Campus, Wollongong, NSW 2500, Australia

来源：

ANALYST | 2020年 / 145卷 / 21期

基金：

澳大利亚研究理事会;

关键词：

MICROFLUIDIC DEVICE; AMBIENT CONDITIONS; SMALL MOLECULES; ION-SOURCE; SPRAY; VERSATILE; BLOOD; MECHANISMS; SEPARATION; BIOSENSOR;

D O I：

10.1039/d0an01344g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The combination of a thread-based electrofluidic analytical device and desorption electrospray ionization mass-spectrometry (DESI-MS) was investigated for the separation and concentration of proteins. The combination delivered a low-cost novel approach for sample pretreatment and target focusing, with direct "on-thread" ambient mass spectrometry detection. For this purpose, a platform for thread-based isoelectric focusing (TB-IEF) was 3D-printed, optimised, and applied to the separation and focusing of three model proteins. Successful separation and focusing was achieved within 30 min. The TB-IEF device was coupled with DESI-MS by direct exposure of the focused solutes on the dried thread to the DESI source. As a proof-of-concept, a 10-fold increase in the DESI-MS response for insulin was achieved following the TB-IEF preconcentration, whilst simultaneously isolating the target solutes from their sample matrix.

引用

页码：6928 / 6936

页数：9

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