Quantitative analysis of changes in salivary mutans streptococci after orthodontic treatment

被引:31
作者
Jung, Woo-Sun [1 ,2 ]
Kim, Ho [3 ,4 ]
Park, So-Yoon [1 ,2 ]
Cho, Eun-Jung [1 ,2 ]
Ahn, Sug-Joon [1 ,2 ]
机构
[1] Seoul Natl Univ, Sch Dent, Dent Res Inst, Seoul 110768, South Korea
[2] Seoul Natl Univ, Dept Orthodont, Seoul 110768, South Korea
[3] Seoul Natl Univ, Grad Sch Publ Hlth, Dept Biostat, Seoul 110768, South Korea
[4] Seoul Natl Univ, Inst Hlth & Environm, Seoul 110768, South Korea
基金
新加坡国家研究基金会;
关键词
POLYMERASE-CHAIN-REACTION; MESSENGER-RNA EXPRESSION; DENTURE BIOFILM ACCUMULATION; PRESCHOOL-CHILDREN; DENTAL PLAQUE; ORAL HYGIENE; CARIES; SOBRINUS; APPLIANCES; PCR;
D O I
10.1016/j.ajodo.2013.12.025
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction: The purpose of this study was to analyze the initial changes in salivary mutans streptococci levels after orthodontic treatment with fixed appliances. Methods: Our subjects consisted of 58 adults. Whole saliva and simplified oral hygiene index values were obtained at 4 time points: at debonding (T1), 1 week after debonding (T2), 5 weeks after debonding (T3), and 13 weeks after debonding (T4). Repeated measures analysis of variance was used to determine the time-related differences in salivary bacterial levels and the simplified oral hygiene index values among the 4 time points after quantifying the salivary levels of Streptococcus mutans, Streptococcus sobrinus, and total bacteria with real-time polymerase chain reaction. Results: Simplified oral hygiene index values and total bacteria significantly decreased, but salivary mutans streptococci levels significantly increased after orthodontic treatment. The amounts of total bacteria in saliva significantly decreased at T3 (T1, T2 > T3, T4), and the simplified oral hygiene index values decreased at T2 (T1. T2, T3, T4). However, salivary S mutans and S sobrinus significantly increased at T3 and T4, respectively (T1, T2 < T3 < T4). Furthermore, the proportion of mutans streptococci to total bacteria significantly increased at T4 (T1, T2, T3 < T4). Conclusions: This study suggests that careful hygienic procedures are needed to reduce the risk for dental caries after orthodontic treatment, despite overall improved oral hygiene status.
引用
收藏
页码:603 / 609
页数:7
相关论文
共 30 条
[1]   Roles of salivary proteins in the adherence of oral streptococci to various orthodontic brackets [J].
Ahn, SJ ;
Kho, HS ;
Lee, SW ;
Nahm, DS .
JOURNAL OF DENTAL RESEARCH, 2002, 81 (06) :411-415
[2]   Evaluation of home disinfection protocols for acrylic baseplates of removable orthodontic appliances: A randomized clinical investigation [J].
Alves Peixoto, Iza Teixeira ;
Enoki, Carla ;
Ito, Izabel Yoko ;
Nakane Matsumoto, Mirian Aiko ;
Nelson-Filho, Paulo .
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, 2011, 140 (01) :51-57
[3]   Ecological study of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus spp. at sub-sites from approximal dental plaque from children [J].
Babaahmady, KG ;
Challacombe, SJ ;
Marsh, PD ;
Newman, HN .
CARIES RESEARCH, 1998, 32 (01) :51-58
[4]   Effect of removable orthodontic appliances on oral colonisation by mutans streptococci in children [J].
Batoni, G ;
Pardini, M ;
Giannotti, A ;
Ota, F ;
Giuca, MR ;
Gabriele, M ;
Campa, M ;
Senesi, S .
EUROPEAN JOURNAL OF ORAL SCIENCES, 2001, 109 (06) :388-392
[5]   Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries [J].
Choi, Eun-Jung ;
Lee, Sung-Hoon ;
Kim, Young-Jae .
INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY, 2009, 19 (02) :141-147
[6]   Effect of Chlorhexidine on Denture Biofilm Accumulation [J].
de Andrade, Ingrid Machado ;
Cruz, Patricia C. ;
Silva-Lovato, Cludia H. ;
de Souza, Raphael F. ;
Monteiro Souza-Gugelmin, Maria Cristina ;
Oliveira Paranhos, Helena de Freitas .
JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY, 2012, 21 (01) :2-6
[7]   Therapeutic Vaccine against Streptococcus sobrinus-induced Caries [J].
Dinis, M ;
Tavares, D ;
Fonseca, AJMM ;
Faria, R ;
Ribeiro, A ;
Cabrita, AMS ;
Ferreira, P .
JOURNAL OF DENTAL RESEARCH, 2004, 83 (04) :354-358
[8]   Effect of denture base materials on mRNA expression of the adhesion-associated genes from the Streptococcus mutans biofilms [J].
Dong, C. ;
Zhang, F. -Q. .
JOURNAL OF ORAL REHABILITATION, 2009, 36 (12) :894-901
[9]   Differential and quantitative analyses of mRNA expression of glucosyltransferases from Streptococcus mutans MT8148 [J].
Fujiwara, T ;
Hoshino, T ;
Ooshima, T ;
Hamada, S .
JOURNAL OF DENTAL RESEARCH, 2002, 81 (02) :109-113
[10]   SIMPLIFIED ORAL HYGIENE INDEX [J].
GREENE, JC ;
VERMILLION, JR .
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 1964, 68 (01) :7-&