Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation

被引：73

作者：

Zhu, Bao-Hua ^{[1
]}

Shi, Hong-Ping ^{[1
]}

Yang, Guan-Pin ^{[2
]}

Lv, Na-Na ^{[1
]}

Yang, Miao ^{[1
]}

Pan, Ke-Hou ^{[1
]}

机构：

[1] Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China

[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

来源：

NEW BIOTECHNOLOGY | 2016年 / 33卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

TRANSFORMATION; EXPRESSION;

D O I：

10.1016/j.nbt.2015.06.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of the suppression of UDP-glucose pyrophosphorylase (UGPase) on chrysolaminaran biosynthesis and carbon allocation were investigated in Phaeodactylum tricornutum. The 69% decrease in UGPase activity was accompanied by a 4.89 fold reduction in Ugp transcript abundance. Inactivation of UGPase in P. tricornutum led to a significant decrease in chrysolaminaran content and an increase in lipid synthesis. These findings suggest that UGPase is a rate-limiting enzyme and may play an important role in chrysolaminarin biosynthesis and carbon allocation. Our results support a theoretical deduction that Ugp is a good candidate for improving lipid synthesis in diatoms.

引用

页码：237 / 244

页数：8

共 28 条

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