Development of SYBR Green I-Based One-Step Real Time RT-PCR Assay for Quantifying Southern rice black- streaked dwarf virus in Rice

被引：14

作者：

Zhou, Tong ^{[1
]}

Du, Linlin ^{[1
]}

Lan, Ying ^{[1
]}

Sun, Feng ^{[1
]}

Fan, Yongjian ^{[1
]}

Zhou, Yijun ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Plant Protect Diag & Detect, Ctr Plant Virus Dis, Nanjing 210014, Jiangsu, Peoples R China

来源：

JOURNAL OF PHYTOPATHOLOGY | 2014年 / 162卷 / 01期

关键词：

one-step real time RT-PCR; quantitation; Southern rice black-streaked dwarf virus; SYBR Green I; DISEASE;

D O I：

10.1111/jph.12152

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Southern rice black-streaked dwarf virus (SRBSDV) causes southern rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses of crops in Southeast Asia. We report here a SYBR Green I-based One-Step Real Time RT-PCR assay for quantifying SRBSDV in rice rapidly and accurately. Primers used for assay were designed from the conserved sequence in S9 RNA among SRBSDV isolates. The RNA standards targeting the S9 region were obtained by transcription in vitro for generation of a standard curve. The assay developed in this study was found to be 100 times more sensitive than the conventional RT-PCR for SRBSDV detection. The primers were very specific for SRBSDV. This study clearly demonstrated the potential usefulness of developed assay for detection and quantitation of SRBSDV in rice samples.

引用

页码：26 / 32

页数：7