Analysis of volatile organic compounds (VOCs) in breath holds great promise for noninvasive diagnostic applications. However, concentrations of VOCs in breath may change quickly, and actual and previous uptakes of exogenous substances, especially in the clinical environment, represent crucial issues. We therefore adapted proton-transfer-reaction-time-of-flight-mass spectrometry for real time breath analysis in the clinical environment. For reasons of medical safety, a 6 m long heated silcosteel transfer line connected to a sterile mouth piece was used for breath sampling from spontaneously breathing volunteers and mechanically ventilated patients. A time resolution of 200 ms was applied. Breath from mechanically ventilated patients was analyzed immediately after cardiac surgery. Breath from 32 members of staff was analyzed in the post anesthetic care unit (PACU). In parallel, room air was measured continuously over 7 days. Detection limits for breath-resolved real time measurements were in the high pptV/low ppbV range. Assignment of signals to alveolar or inspiratory phases was done automatically by a matlab-based algorithm. Quickly and abruptly occurring changes of patients' clinical status could be monitored in terms of breath-to-breath variations of VOC (e.g. isoprene) concentrations. In the PACU, room air concentrations mirrored occupancy. Exhaled concentrations of sevoflurane strongly depended on background concentrations in all participants. In combination with an optimized inlet system, the high time and mass resolution of PTR-ToF-MS provides optimal conditions to trace quick changes of breath VOC profiles and to assess effects from the clinical environment.
机构:
Natl Ctr Atmospher Res, NCAR Earth Syst Lab, Boulder, CO 80307 USAFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Karl, Thomas
Probst, Michael
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Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, AustriaFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Probst, Michael
Ismailova, Oksana
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Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, AustriaFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Ismailova, Oksana
Winkler, Paul M.
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Natl Ctr Atmospher Res, NCAR Earth Syst Lab, Boulder, CO 80307 USAFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Winkler, Paul M.
Soukoulis, Christos
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Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Soukoulis, Christos
Aprea, Eugenio
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Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Aprea, Eugenio
Maerk, Tilmann D.
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Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, AustriaFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Maerk, Tilmann D.
Gasperi, Flavia
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Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Gasperi, Flavia
Biasioli, Franco
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Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
机构:
Natl Ctr Atmospher Res, NCAR Earth Syst Lab, Boulder, CO 80307 USAFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Karl, Thomas
Probst, Michael
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机构:
Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, AustriaFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Probst, Michael
Ismailova, Oksana
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h-index: 0
机构:
Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, AustriaFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Ismailova, Oksana
Winkler, Paul M.
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h-index: 0
机构:
Natl Ctr Atmospher Res, NCAR Earth Syst Lab, Boulder, CO 80307 USAFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Winkler, Paul M.
Soukoulis, Christos
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h-index: 0
机构:
Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Soukoulis, Christos
Aprea, Eugenio
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h-index: 0
机构:
Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Aprea, Eugenio
Maerk, Tilmann D.
论文数: 0引用数: 0
h-index: 0
机构:
Leopold Franzens Univ Innsbruck, Inst Ionenphys & Angew Phys, A-6020 Innsbruck, AustriaFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Maerk, Tilmann D.
Gasperi, Flavia
论文数: 0引用数: 0
h-index: 0
机构:
Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy
Gasperi, Flavia
Biasioli, Franco
论文数: 0引用数: 0
h-index: 0
机构:
Fdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, ItalyFdn Edmund Mach, IASMA Res & Innovat Ctr, Food Qual & Nutr Area, I-38010 S Michele A A, Italy