Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP

被引：389
作者：
Fanello, C
Santolamazza, F
della Torre, A
机构：
[1] Univ Roma La Sapienza, Dipartimento Sci Sanita Publ, Sez Parassitol, I-00185 Rome, Italy
[2] London Sch Hyg & Trop Med, London WC1, England
来源：
MEDICAL AND VETERINARY ENTOMOLOGY | 2002年 / 16卷 / 04期
关键词：
Anopheles gambiae complex; malaria vectors; molecular markers; mosquito identification; PCR-RFLP; sibling species; Africa;
D O I：
10.1046/j.1365-2915.2002.00393.x
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto , we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.
引用
收藏
页码：461 / 464
页数：4
相关论文
共 13 条
  • [1] Distribution of African malaria mosquitoes belonging to the Anopheles gambiae complex
    Coetzee, M
    Craig, M
    le Sueur, D
    [J]. PARASITOLOGY TODAY, 2000, 16 (02): : 74 - 77
  • [2] CHROMOSOMAL INVERSION INTERGRADATION AND INCIPIENT SPECIATION IN ANOPHELES-GAMBIAE
    COLUZZI, M
    PETRARCA, V
    DIDECO, MA
    [J]. BOLLETTINO DI ZOOLOGIA, 1985, 52 (1-2): : 45 - 63
  • [3] CHROMOSOMAL DIFFERENTIATION AND ADAPTATION TO HUMAN ENVIRONMENTS IN THE ANOPHELES-GAMBIAE COMPLEX
    COLUZZI, M
    SABATINI, A
    PETRARCA, V
    DIDECO, MA
    [J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1979, 73 (05) : 483 - 497
  • [4] COLUZZI M, 2002, IN PRESS SCIENCE
  • [5] della Torre A, 2001, INSECT MOL BIOL, V10, P9, DOI 10.1046/j.1365-2583.2001.00235.x
  • [6] Speciation within Anopheles gambiae -: the glass is half full
    della Torre, A
    Costantini, C
    Besansky, NJ
    Caccone, A
    Petrarca, V
    Powell, JR
    Coluzzi, M
    [J]. SCIENCE, 2002, 298 (5591) : 115 - 117
  • [7] Molecular characterization of ribosomal DNA polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s.
    Favia, G
    Lanfrancotti, A
    Spanos, L
    Sidén-Kiamos, I
    Louis, C
    [J]. INSECT MOLECULAR BIOLOGY, 2001, 10 (01) : 19 - 23
  • [8] Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation
    Favia, G
    dellaTorre, A
    Bagayoko, M
    Lanfrancotti, A
    Sagnon, N
    Toure, YT
    Coluzzi, M
    [J]. INSECT MOLECULAR BIOLOGY, 1997, 6 (04) : 377 - 383
  • [9] PCR assay for identification of Anopheles quadriannulatus species B from Ethiopia and other sibling species of the Anopheles gambiae complex
    Fettene, M
    Koekemoer, LL
    Hunt, RH
    Coetzee, M
    [J]. MEDICAL AND VETERINARY ENTOMOLOGY, 2002, 16 (02) : 214 - 217
  • [10] Attempts to molecularly distinguish cryptic taxa in Anopheles gambiae s.s.
    Gentile, G
    Slotman, M
    Ketmaier, V
    Powell, JR
    Caccone, A
    [J]. INSECT MOLECULAR BIOLOGY, 2001, 10 (01) : 25 - 32
12