Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

被引：2
作者：
Sendra, Miquel [1 ]
Manes, Jorge [1 ]
Dominguez, Jorge N. [2 ,3 ]
Torres, Miguel [1 ]
机构：
[1] Ctr Nacl Invest Cardiovasc CNIC, Cardiovasc Regenerat Program, Madrid, Spain
[2] Univ Jaen, Dept Expt Biol, Cardiovasc Dev Grp, Jaen, Spain
[3] Fdn Medina, Granada, Spain
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2022年 / 185期
基金：
欧盟地平线“2020”;
关键词：
ALLOCATION; CULTURE; HEART;
D O I：
10.3791/64273
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The first steps of heart development imply drastic changes in cell behavior and differentiation. While analysis of fixed embryos allows studying in detail specific developmental stages in a still snapshot, live imaging captures dynamic morphogenetic events, such as cell migration, shape changes, and differentiation, by imaging the embryo as it develops. This complements fixed analysis and expands the understanding of how organs develop during embryogenesis. Despite its advantages, live imaging is rarely used in mouse models because of its technical challenges. Early mouse embryos are sensitive when cultured ex vivo and require efficient handling. To facilitate a broader use of live imaging in mouse developmental research, this paper presents a detailed protocol for two-photon live microscopy that allows long-term acquisition in mouse embryos. In addition to the protocol, tips are provided on embryo handling and culture optimization. This will help understand key events in early mouse organogenesis, enhancing the understanding of cardiovascular progenitor biology.
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页数：11
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