Circulating giant macrophages as a potential biomarker of solid tumors

被引：216

作者：

Adams, Daniel L. ^{[1
]}

Martin, Stuart S. ^{[2
]}

Alpaugh, R. Katherine ^{[3
]}

Charpentier, Monica ^{[2
]}

Tsai, Susan ^{[4
]}

Bergan, Raymond C. ^{[5
]}

Ogden, Irene M. ^{[5
]}

Catalona, William ^{[5
]}

Chumsri, Saranya ^{[2
]}

Tang, Cha-Mei ^{[6
]}

Cristofanilli, Massimo ^{[7
]}

机构：

[1] Creatv MicroTech Inc, Rockville, MD 20850 USA

[2] Univ Maryland, Greenebaum Canc Ctr, Baltimore, MD 21201 USA

[3] Fox Chase Canc Ctr, Protocol Support Lab, Philadelphia, PA 19111 USA

[4] Med Coll Wisconsin, Milwaukee, WI 53226 USA

[5] Northwestern Univ, Robert H Lurie Canc Ctr, Chicago, IL 60611 USA

[6] Creatv MicroTech Inc, Potomac, MD 20854 USA

[7] Thomas Jefferson Univ Hosp, Philadelphia, PA 19107 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 09期

基金：

美国国家卫生研究院;

关键词：

liquid biopsy; blood cell biomarkers; cancer metastasis; cancer biomarker; cancer screening; PERIPHERAL-BLOOD; BREAST-CANCER; CELLS; IDENTIFICATION; METASTASIS; MEGAKARYOCYTES; PROGENITORS; MIGRATION; LINEAGE;

D O I：

10.1073/pnas.1320198111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tumor-associated macrophages (TAMs) derived from primary tumors are believed to facilitate circulating tumor cell (CTC) seeding of distant metastases, but the mechanisms of these processes are poorly understood. Although many studies have focused on the migration of CTCs, less attention has been given to TAMs that, like CTCs, derive from tumor sites. Using precision microfilters under low-flow conditions, we isolated circulating cancer-associated macrophage-like cells (CAMLs) from the peripheral blood of patients with breast, pancreatic, or prostate cancer. CAMLs, which are not found in healthy individuals, were found to express epithelial, monocytic, and endothelial protein markers and were observed bound to CTCs in circulation. These data support the hypothesis that disseminated TAMs can be used as a biomarker of advanced disease and suggest that they have a participatory role in tumor cell migration.

引用

页码：3514 / 3519

页数：6

共 26 条

[1] The systematic study of circulating tumor cell isolation using lithographic microfilters [J].