Comparative virulence of Candida auris with Candida haemulonii, Candida glabrata and Candida albicans in a murine model

被引：107

作者：

Fakhim, Hamed ^{[1
,2
]}

Vaezi, Afsane ^{[3
,4
]}

Dannaoui, Eric ^{[5
]}

Chowdhary, Anuradha ^{[6
]}

Nasiry, Davood ^{[7
]}

Faeli, Leila ^{[3
,4
]}

Meis, Jacques F. ^{[8
,9
]}

Badali, Hamid ^{[4
]}

机构：

[1] Urmia Univ Med Sci, Fac Med, Dept Med Parasitol & Mycol, Orumiyeh, Iran

[2] Urmia Univ Med Sci, Cellular & Mol Res Ctr, Orumiyeh, Iran

[3] Mazandaran Univ Med Sci, Student Res Comm, Sari, Iran

[4] Mazandaran Univ Med Sci, Dept Med Mycol, Invas Fungi Res Ctr, Sch Med, Sari, Iran

[5] Univ Paris 05, Hop Europeen Georges Pompidou, AP HP, Unite Parasitol Mycol,Serv Microbiol,Fac Med, Paris, France

[6] Univ Delhi, Vallabhbhai Patel Chest Inst, Dept Med Mycol, Delhi, India

[7] Shahid Beheshti Univ Med Sci, Dept Biol & Anat Sci, Student Res Comm, Sch Med, Tehran, Iran

[8] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, ECMM Excellence Ctr Med Mycol, Nijmegen, Netherlands

[9] Ctr Expertise Mycol Radboudumc CWZ Nijmegen, Nijmegen, Netherlands

来源：

MYCOSES | 2018年 / 61卷 / 06期

关键词：

Candida auris; disseminated candidiasis; murine model; pathogenicity; survival study; tissue burden; ANTIFUNGAL SUSCEPTIBILITY; CLONAL STRAIN; INFECTIONS; FUNGEMIA; LESSONS;

D O I：

10.1111/myc.12754

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The incidence of invasive fungal infections (IFIs) caused by uncommon Candida species with diverse virulence and susceptibility profiles has increased in recent years. Due to scarce clinical and experimental data on the pathogenicity of Candida auris, the aim of this study was to evaluate and compare the virulence of two rare clinically relevant species, C.auris and Candida haemulonii with Candida glabrata and Candida albicans in an immunocompetent murine model of disseminated infection. Immunocompetent ICR female mice were infected with three inoculum sizes (1x10(5), 1x10(6) and 1x10(7) CFU/mouse) of two C.auris strains and one isolate of C.haemulonii, C.glabrata and C.albicans. Tissue burden on days 5 and 10 postchallenge and mortality rate were used as virulence markers. A high virulence was found for C.albicans, followed by C.auris, C. glabrata and C.haemulonii, respectively. Candidaalbicans showed high virulence with a medium survival time of 9.5 days for mice infected with 1x10(7) CFU/mouse. For inocula at 1x10(6) and 1x10(7) CFU/mouse, there were significant differences in fungal burden at day 10 between C.albicans, C.auris and C.glabrata isolates compared with C.haemulonii (P<.0001). Overall, no significant differences between C.albicans with C.auris and C. glabrata were observed in mice infected with three different inocula (P>.05). In general, the highest fungal load of all isolates was detected in kidney followed by spleen, liver and lung tested with three different inocula on the two different experimental days. Histopathological examination revealed the abundant presence of yeast cells with pseudohyphae for C.albicans and only yeast cells for C.auris, C.glabrata and C.haemulonii, in all the kidney tissue samples. In conclusion, C.albicans is a highly virulent opportunistic fungus, as the clinical and experimental data demonstrate, and also our results demonstrate a low virulence of C.haemulonii in immunocompetent animals. Altogether, this study highlights the pathogenic potential of C.auris.

引用

页码：377 / 382

页数：6

共 33 条

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