Wavefront correction in two-photon microscopy with a multi-actuator adaptive lens

被引：24

作者：

Bueno, Juan M. ^{[1
]}

Skorsetz, Martin ^{[1
]}

Bonora, Stefano ^{[2
]}

Artal, Pablo ^{[1
]}

机构：

[1] Univ Murcia, Inst Univ Invest Opt & Nanofis, Lab Opt, Campus Espinardo Ed 34, E-30100 Murcia, Spain

[2] CNR Inst Photon & Nanotechnol, Via Trasea 7, I-35131 Padua, Italy

来源：

OPTICS EXPRESS | 2018年 / 26卷 / 11期

基金：

欧洲研究理事会;

关键词：

FLUORESCENCE MICROSCOPY; MULTIPHOTON MICROSCOPY; COMPENSATION TECHNIQUE; ABERRATION CORRECTION; OPTICS; RESOLUTION; TISSUE; IMPLEMENTATION; IMAGES; LIGHT;

D O I：

10.1364/OE.26.014278

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A multi-actuator adaptive lens (AL) was incorporated into a multi-photon (MP) microscope to improve the quality of images of thick samples. Through a hill-climbing procedure the AL corrected for the specimen-induced aberrations enhancing MP images. The final images hardly differed when two different metrics were used, although the sets of Zernike coefficients were not identical. The optimized MP images acquired with the AL were also compared with those obtained with a liquid-crystal-on-silicon spatial light modulator. Results have shown that both devices lead to similar images, which corroborates the usefulness of this AL for MP imaging. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：14278 / 14287

页数：10

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