Effect of Exogenous Ethylene (ET) on Regulating Carotenogenesis Expression and Astaxanthin Accumulation in Haematococcus pluvialis

被引：1
作者：
Gao, Zhengquan [1 ]
Meng, Chunxiao [1 ]
Gao, Hongzheng [1 ]
Ye, Naihao [2 ]
Zhang, Xiaowen [2 ]
Su, Yuanfeng [1 ]
Zhao, Yuren [1 ]
Wang, Yuanyuan [1 ]
机构：
[1] Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
[2] Chinese Acad Fishery Sci, Yellow Sea Fishery Res Inst, Qingdao 266071, Peoples R China
来源：
JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2013年 / 7卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Haematococcus pluvialis; Astaxanthin; Ethylene (ET); Regulation; Carotenoid genes; Real-time fluorescence quantitative PCR (qRT-PCR); BETA-CAROTENE KETOLASE; GENE-EXPRESSION; STRESS; CHLOROPHYCEAE; BIOSYNTHESIS; PROMOTER; ACID;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The freshwater eukaryotic unicellular alga Haematococcus pluvialis is one of the best sources to produce natural astaxanthin in nature. However, the regulatory role of carotenogenesis leading to astaxanthin formation is not well understood. In this study, the effects of ET on transcriptional expressions of eight carotenoid genes in H. pluvialis were measured using qRT-PCR. Results showed that eight carotenogenic genes were up-regulated by ET with different expression profiles. Astaxanthin accumulation was promoted efficiently by ET, which might result from the up-regulating of ipi-1, ipi-2, pds, lyc, bkt, crtR-b and crtO at the transcriptional level, and by psy at both transcriptional and post-transcriptional levels. Moreover, fourteen proteins were expressed differentially in ET treatments compared to controls. Furthermore, algal cell division accompanying accompanied by astaxanthin accumulation for the first time during the whole course of ethylene induction. The knowledge in the present paper might help to optimize the physiological conditions needed to produce a high yield of astaxanthin with ET.
引用
收藏
页码：2503 / 2511
页数：9
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