Genetic diversity of Pneumocystis jirovecii in colonized Cuban infants and toddlers

被引：17
作者：
Monroy-Vaca, Ernesto X. [1 ]
de Armas, Yaxsier [1 ]
Illnait-Zaragozi, Maria T. [1 ]
Diaz, Raul [1 ]
Torano, Gilda [1 ]
Vega, Dania [2 ]
Alvarez-Lam, Ileana [3 ]
Calderon, Enrique J. [4 ,5 ]
Stensvold, Christen R. [6 ]
机构：
[1] Inst Trop Med Pedro Kouri, Dept Bacteriol & Mycol, Havana, Cuba
[2] Pediat Hosp Juan Manuel Marquez, Havana, Cuba
[3] Pediat Hosp William Soler, Havana, Cuba
[4] Hosp Univ Virgen del Rocio, Inst Biomed Sevilla, Seville, Spain
[5] Hosp Univ Virgen del Rocio, CIBER Epidemiol & Salud Publ, Seville, Spain
[6] Statens Serum Inst, Dept Microbiol & Infect Control, Unit Mycol & Parasitol, DK-2300 Copenhagen, Denmark
来源：
INFECTION GENETICS AND EVOLUTION | 2014年 / 22卷
关键词：
Pneumocystis; Colonization; Genotypes; Infants; Toddlers; Cuba; F-SP; HOMINIS; MULTILOCUS GENOTYPING PROFILES; HIV-INFECTED PATIENTS; RIBOSOMAL-RNA; IMMUNOCOMPETENT INFANTS; CARINII; PNEUMONIA; SEQUENCES; DNA; POLYMORPHISMS;
D O I：
10.1016/j.meegid.2013.12.024
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Pneumocystis jirovecii is a leading cause of opportunistic infections among immunocompromised patients. The aim of this study was to determine the genetic diversity of P. jirovecii from colonized Cuban infants and toddlers by analysis of four genetic loci: mitochondrial large subunit (mtLSU) rRNA, cytochrome b (CYB), superoxide dismutase (SOD) and b-tubulin (b-tub). We determined the multilocus profiles based on concatenated genotype data (multilocus genotype; MLG) and nucleotide sequences (multilocus sequence analysis; MLSA) respectively, calculated the discriminatory power of each analysis, and investigated possible associations with demographic and clinical data. Sixteen of 51 PCR-positive nasopharyngeal swab specimens (years 2010-2013) with high P. jirovecii load were selected for downstream analysis. In mixed allelic profiles all genotypes/nucleotide sequence patterns were considered separately. All samples could be genotyped based on mtLSU, CYB and b-tub locus. However, the SOD locus could be successfully amplified in only 7/16 (44%) specimens. Eight different P. jirovecii MLGs were identified among the 16 cases and eight samples presented identical MLG (MLG 1). Seventeen MLSA profiles were distinguished. No statistical association between genotypes or MLGs and demographic or clinical data could be identified. For MLSA the higher discriminatory power (S = 0.976) was observed. The combination of mtLSU, CYB and b-tub loci proved to be useful for molecular epidemiology studies of P. jirovecii. A total of 17 different MLSA profiles observed in 16 specimens indicated high genetic variability of P. jirovecii circulating in colonized Cuban infants and toddlers. (c) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：60 / 66
页数：7
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