Pseudomonas aeruginosa Biofilm Growth Inhibition on Medical Plastic Materials by Immobilized Esterases and Acylase

被引:14
作者
Kisch, Johannes Martin [1 ]
Utpatel, Christian [2 ]
Hilterhaus, Lutz [1 ]
Streit, Wolfgang R. [2 ]
Liese, Andreas [1 ]
机构
[1] Hamburg Univ Technol, Inst Tech Biocatalysis, D-21071 Hamburg, Germany
[2] Univ Hamburg, Biozentrum Klein Flottbek, Abt Mikrobiol & Biotechnol, D-22609 Hamburg, Germany
关键词
biofilms; immobilization; lactones; polymer coating; quorum quenching; QUORUM; VIRULENCE; SIGNAL; COMMUNICATION; ANTIBIOTICS; SPECIFICITY; INFECTIONS; EXPRESSION; PREVENTION; SURFACES;
D O I
10.1002/cbic.201400023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Biofilms are matrix-encapsulated cell aggregates that cause problems in technical and health-related areas; for example, 65% of all human infections are biofilm associated. This is mainly due to their ameliorated resistance against antimicrobials and immune systems. Pseudomonas aeruginosa, a biofilm-forming organism, is commonly responsible for nosocomial infections. Biofilm development is partly mediated by signal molecules, such as acyl-homoserine lactones (AHLs) in Gram-negative bacteria. We applied horse liver esterase, porcine kidney acylase, and porcine liver esterase; these can hydrolyze AHLs, thereby inhibiting biofilm formation. As biofilm infections are often related to foreign material introduced into the human body, we immobilized the enzymes on medical plastic materials. Biofilm formation was quantified by Crystal Violet staining and confocal laser scanning microscopy, revealing up to 97% (on silicone), 54% (on polyvinyl chloride), and 77% (on polyurethane) reduced biomass after 68 h growth.
引用
收藏
页码:1911 / 1919
页数:9
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