A Simple Colorimetric Enzymatic-Assay, based on immobilization of acetylcholinesterase by adsorption, for sensitive detection of organophosphorus insecticides in olive oil

被引：19

作者：

Ben Oujji, Najwa ^{[1
,2
]}

Bakas, Idriss ^{[1
,2
]}

Istamboulie, Georges ^{[1
]}

Ait-Ichou, Ihya ^{[2
]}

Ait-Addi, Elhabib ^{[2
]}

Rouillon, Regis ^{[1
]}

Noguer, Thierry ^{[1
]}

机构：

[1] Univ Perpignan, IMAGES Lab, IMAGES EA4218, F-66860 Perpignan, France

[2] Univ Ibn Zohr, AQUAMAR, Photocatalysis & Environm Team, Chem Dept,Fac Sci, Agadir, Morocco

来源：

FOOD CONTROL | 2014年 / 46卷

关键词：

Electric eel acetylcholinesterase; Colorimetric Enzymatic-Assay; Olive oil; Organophosphorus insecticides; BIOSENSORS; PESTICIDES; BIOASSAY; RESIDUES;

D O I：

10.1016/j.foodcont.2014.05.006

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Over the last decades, cholinesterase (ChE) biosensors/bioassays have emerged as an ultra sensitive and rapid technique for pesticides detection in environmental monitoring, food and quality control. In this work a Simple Colorimetric Enzymatic-Assay for the sensitive detection of three organophosphorus insecticides widely used in the treatment of olive trees: Malathion, Dimethoate and Methidathion in their oxidized form was developed. The systems are based on the immobilization of 0.3 mIU of electric eel acetylcholinesterase by adsorption on MaxiSorp (TM) microplates, and tested using standard solutions and real samples of olive oil. The developed colorimetric tests showed good analytical performances with limits of detection as low as 10(-9) M for the widely used pesticide malaoxon (oxidized malathion), as well as a good reproducibility, good operational and storage stability and they can be applied directly on olive oil real samples without a laborious pre-treatment and after a simple liquid liquid extraction, which demonstrate the potentiality of this technique to be used for commercial purpose. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：75 / 80

页数：6

共 18 条

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