Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell-Mediated Mechanism

被引：11

作者：

Rahman, Mizanur ^{[1
]}

Steuer, Johnny ^{[2
]}

Gillgren, Peter ^{[2
]}

Vegvari, Akos ^{[3
]}

Liu, Anquan ^{[1
]}

Frostegard, Johan ^{[1
]}

机构：

[1] Karolinska Inst, Inst Environm Med, Stockholm, Sweden

[2] Karolinska Inst, Inst Clin Sci & Educ, Dept Surg, Sect Vasc Surg,Sodersjukhuset, Stockholm, Sweden

[3] Karolinska Inst, Dept Med Biochem & Biophys, Div Chem 1, Biomed, Stockholm, Sweden

来源：

JACC-BASIC TO TRANSLATIONAL SCIENCE | 2019年 / 4卷 / 04期

基金：

瑞典研究理事会;

关键词：

atherosclerosis; dendritic cells; T cells; malondialdehyde; oxidized low-density lipoprotein; LOW-DENSITY-LIPOPROTEIN; SYSTEMIC-LUPUS-ERYTHEMATOSUS; HEAT-SHOCK-PROTEIN; IMMUNE-ACTIVATION; SERUM ANTIBODIES; DEFICIENT MICE; HIGH TITERS; ASSOCIATION; LYMPHOCYTES; INDUCTION;

D O I：

10.1016/j.jacbts.2019.03.009

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by mass spectrometry. MDA-HSA induced a pro-inflammatory DC-mediated T-cell activation and also a strong direct effect on T cells, inhibited by an inhibitor of oxidative stress and antibodies against MDA. Atherogenic heat shock protein-60 was strongly induced in T cells activated by MDA-HSA. Two peptide modifications in atherosclerotic patients' HSA were similar to those present in in vitro MDA-modified HSA. (C) 2019 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation.

引用

页码：480 / 494

页数：15

共 3 条

[1] Induction of Dendritic Cell-Mediated Activation of T Cells From Atherosclerotic Plaques by Human Heat Shock Protein 60
Rahman, Mizanur
Steuer, Johnny
Gillgren, Peter
Hayderi, Assim
Liu, Anquan
Frostegard, Johan
JOURNAL OF THE AMERICAN HEART ASSOCIATION, 2017, 6 (11):
[2] Human C-reactive protein activates monocyte-derived dendritic cells and induces dendritic cell-mediated T-cell activation
Van Vre, Emily A.
Bult, Hidde
Hoymans, Vicky Y.
Van Tendeloo, Viggo F. I.
Vrints, Christiaan J.
Bosmans, Johan M.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2008, 28 (03) : 511 - 518
[3] Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand
Wesa A.
Galy A.
BMC Immunology, 3 (1)

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