Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution

被引：113

作者：

Zhao, Yan-Jie ^{[1
,2
]}

Yu, Ting-Ting ^{[1
,2
]}

Zhang, Chao ^{[1
,2
]}

Li, Zhao ^{[1
,2
]}

Luo, Qing-Ming ^{[1
,2
]}

Xu, Tong-Hui ^{[1
,2
]}

Zhu, Dan ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect, Wuhan 430074, Peoples R China

[2] Huazhong Univ Sci & Technol, Sch Engn Sci, Collaborat Innovat Ctr Biomed Engn, MoE Key Lab Biomed Photon, Wuhan 430074, Peoples R China

来源：

LIGHT-SCIENCE & APPLICATIONS | 2018年 / 7卷

基金：

中国国家自然科学基金;

关键词：

cranial window; dendritic spines; optical clearing; synaptic resolution; two-photon imaging; LONG-TERM; CRANIAL WINDOW; TISSUE; TRANSPARENT; NEOCORTEX; ACCESS; ORGANS;

D O I：

10.1038/lsa.2017.153

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here, we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy, this technique allowed us to repeatedly image neurons, microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety, this method holds great promise for application in neuroscience research.

引用

页码：17153 / 17153

页数：9

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