A droplet microfluidic approach to single-stream nucleic acid isolation and mutation detection

被引：22

作者：

Shin, Dong Jin ^{[1
]}

Zhang, Yi ^{[1
]}

Wang, Tza-Huei ^{[1
,2
,3
,4
]}

机构：

[1] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD 21218 USA

[3] Inst NanoBioTechnol, Baltimore, MD USA

[4] Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA

来源：

MICROFLUIDICS AND NANOFLUIDICS | 2014年 / 17卷 / 02期

基金：

美国国家科学基金会;

关键词：

Droplet microfluidics; Droplet PCR; Melting curve analysis; Mutation detection; MELTING CURVE ANALYSIS; MAGNETIC MANIPULATION; DNA; CHIP; DIAGNOSIS; PLATFORM; PCR;

D O I：

10.1007/s10404-013-1305-7

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

In this work, a droplet microfluidic platform for genetic mutation detection from crude biosample is described. Single-stream integration of nucleic acid isolation and amplification is realized on a simple fluidic cartridge. Subsequent DNA melting curve is employed with signal normalizing algorithm to differentiate heterozygous K-ras codon 12 c.25G > A mutant from the wild type. This technique showcases an alternative to modular bench-top approaches for genetic mutation screening, which is of interest to decentralized diagnostic platforms.

引用

页码：425 / 430

页数：6

共 23 条

[1] Topography-assisted electromagnetic platform for blood-to-PCR in a droplet [J].

Chiou, Chi-Han ;

Shin, Dong Jin ;

Zhang, Yi ;

Wang, Tza-Huei .

BIOSENSORS & BIOELECTRONICS, 2013, 50 :91-99

[2] A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability [J].

Easley, Christopher J. ;

Karlinsey, James M. ;

Bienvenue, Joan M. ;

Legendre, Lindsay A. ;

Roper, Michael G. ;

Feldman, Sanford H. ;

Hughes, Molly A. ;

Hewlett, Erik L. ;

Merkel, Tod J. ;

Ferrance, Jerome P. ;

Landers, James P. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (51) :19272-19277

[3]

GRIFFITHSCHU S, 1984, BLOOD, V64, P296

[4] Mutations of BRAF and KRAS precede the development of ovarian serous borderline tumors [J].

Ho, CL ;

Karman, RJ ;

Dehari, R ;

Wang, TL ;

Shih, LM .

CANCER RESEARCH, 2004, 64 (19) :6915-6918

[5] Two-dimensional magnetic manipulation of microdroplets on a chip as a platform for bioanalytical applications [J].

Lehmann, U. ;

Hadjidj, S. ;

Parashar, V. K. ;

Vandevyver, C. ;

Rida, A. ;

Gijs, M. A. M. .

SENSORS AND ACTUATORS B-CHEMICAL, 2006, 117 (02) :457-463

[6] Sample preparation: the weak link in microfluidics-based biodetection [J].

Mariella, Raymond, Jr. .

BIOMEDICAL MICRODEVICES, 2008, 10 (06) :777-784

[7] Driving forces for DNA adsorption to silica in perchlorate solutions [J].

Melzak, KA ;

Sherwood, CS ;

Turner, RFB ;

Haynes, CA .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 181 (02) :635-644

[8] A digital microfluidic approach to homogeneous enzyme assays [J].

Miller, Elizabeth M. ;

Wheeler, Aaron R. .

ANALYTICAL CHEMISTRY, 2008, 80 (05) :1614-1619

[9] Genotyping of single nucleotide polymorphisms by melting curve analysis using thin film semi-transparent heaters integrated in a lab-on-foil system [J].

Ohlander, Anna ;

Zilio, Caterina ;

Hammerle, Tobias ;

Zelenin, Sergey ;

Klink, Gerhard ;

Chiari, Marcella ;

Bock, Karlheinz ;

Russom, Aman .

LAB ON A CHIP, 2013, 13 (11) :2075-2082

[10] An integrated microfluidic device for influenza and other genetic analyses [J].

Pal, R ;

Yang, M ;

Lin, R ;

Johnson, BN ;

Srivastava, N ;

Razzacki, SZ ;

Chomistek, KJ ;

Heldsinger, DC ;

Haque, RM ;

Ugaz, VM ;

Thwar, PK ;

Chen, Z ;

Alfano, K ;

Yim, MB ;

Krishnan, M ;

Fuller, AO ;

Larson, RG ;

Burke, DT ;

Burns, MA .

LAB ON A CHIP, 2005, 5 (10) :1024-1032

← 1 2 3 →