Effect of sodium ferulate on IL-8 expression induced by Ox-LDL in human umbilical vein endothelial cells

被引：0

作者：

Tao, Junliang ^{[1
,2
]}

Zhang, Dongxian ^{[2
]}

Zhang, Dongyi ^{[3
]}

Man, Yonghong ^{[2
]}

Bi, Yongyi ^{[1
]}

机构：

[1] Wuhan Univ, Sch Publ Hlth, Wuhan, Peoples R China

[2] Nanyang Med Coll, Nanyang, Peoples R China

[3] First Affiliated Hosp Nanyang Med Coll, Nanyang, Peoples R China

来源：

BIOMEDICAL RESEARCH-INDIA | 2017年 / 28卷 / 05期

关键词：

Sodium ferulate; Human umbilical vein endothelial cell; Interleukin-8; Atherosclerosis;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In the present study we try to investigate the effect of Sodium Ferulate (SF) on Oxidized Low-Density Lipoprotein (Ox-LDL) induced IL-8 expression in Human Umbilical Vein Endothelial Cells (HUVEC). HUVECs were cultured in vitro and gene chip assay was used to analyse the regulation of IL-8 gene expression in HUVEC after the stimulation with Ox-LDL and SF+Ox-LDL, respectively. The IL-8 mRNA expression was measured by real-time PCR assay and protein expression by Western and ELISA assay. The results indicated that the expression of IL-8 gene was up-regulated by 6.1 fold after stimulation by Ox-LDL in HUVECs compared with that of the control. However, the expression of IL-8 gene was down-regulated by 3.3 fold after the stimulation by SF+Ox-LDL in HUVECs compared with that of in Ox-LDL treatment group. The real-time PCR results, Western and ELISA results showed that both the mRNA level and protein level of IL-8 increased significantly in Ox-LDL group compared with that of the control, and the expression decreased obviously in SF+Ox-LDL group compared with that of the Ox-LDL group. Take it together; Ox-LDL could induce the expression of IL-8, which could be inhibited by SF. Moreover, SF down-regulated the expression of IL-8, which could reduce the inflammatory response in HUVECs.

引用

页码：1935 / 1938

页数：4

共 13 条

[1] Pseudomonas aeruginosa elastase stimulates ERK signaling pathway and enhances IL-8 production by alveolar epithelial cells in culture [J].

Azghani, AO ;

Baker, JW ;

Shetty, S ;

Miller, EJ ;

Bhat, GJ .

INFLAMMATION RESEARCH, 2002, 51 (10) :506-510

[2] Salvianolic acid B attenuates VCAM-1 and ICAM-1 expression in TNF-α-treated human aortic endothelial cells [J].

Chen, YH ;

Lin, SJ ;

Ku, HH ;

Shiao, MS ;

Lin, FY ;

Chen, JW ;

Chen, YL .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2001, 82 (03) :512-521

[3] MINIMALLY MODIFIED LOW-DENSITY-LIPOPROTEIN INDUCES MONOCYTE CHEMOTACTIC PROTEIN-1 IN HUMAN ENDOTHELIAL-CELLS AND SMOOTH-MUSCLE CELLS [J].

CUSHING, SD ;

BERLINER, JA ;

VALENTE, AJ ;

TERRITO, MC ;

NAVAB, M ;

PARHAMI, F ;

GERRITY, R ;

SCHWARTZ, CJ ;

FOGELMAN, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) :5134-5138

[4] Interleukin-8 as an independent predictor of long-term clinical outcome in patients with coronary artery disease [J].

Inoue, Teruo ;

Komoda, Hiroshi ;

Nonaka, Masako ;

Kameda, Miho ;

Uchida, Toshihiko ;

Node, Koichi .

INTERNATIONAL JOURNAL OF CARDIOLOGY, 2008, 124 (03) :319-325

[5]

Liang LM, 2012, CHIN J LAB DIAG, V16, P1180

[6]

[廖健毅 LIAO Jianyi], 2011, [中华胸心血管外科杂志, Chinese Journal of Thoracic and Cardiovascular Surgery], V27, P221

[7] Apoptotic cell death in atherosclerosis [J].

Littlewood, TD ;

Bennett, MR .

CURRENT OPINION IN LIPIDOLOGY, 2003, 14 (05) :469-475

[8]

[欧阳静萍 Ou-Yang Jingping], 2002, [中国药理学通报, Chinese Pharmacological Bulletin], V18, P207

[9]

[邱雅慧 Qiu YH], 2007, [中国组织工程研究与临床康复, Journal of Clinical Rehabilitative Tissue Engineering Research], V11, P1927

[10] Sodium ferulate inhibits atherosclerogenesis in hyperlipidemia rabbits [J].

Wang, BH ;

Ouyang, JP ;

Liu, YM ;

Yang, JW ;

Wei, L ;

Li, K ;

Yang, HL .

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2004, 43 (04) :549-554

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