Organophosphate hydrolase conjugated UiO-66-NH2 MOF based highly sensitive optical detection of methyl parathion

被引:79
作者
Mehta, Jyotsana [1 ,2 ]
Dhaka, Sarita [1 ]
Paul, Ashok K. [3 ]
Dayananda, Siddavattam [4 ]
Deep, Akash [1 ,2 ]
机构
[1] Cent Sci Instruments Org CSIR CSIO, Div H 1, Nanosci & Nanotechnol Lab, Sect 30C, Chandigarh 160030, India
[2] Acad Sci & Innovat Res AcSIR CSIO, Sect 30C, Chandigarh 160030, India
[3] Desh Bhagat Univ, Mandi Gobindgarh, Punjab, India
[4] Univ Hyderabad, Sch Life Sci, Hyderabad 500046, India
关键词
Zr-MOF; Organophosphate hydrolase; Bioconjugate; Enzyme activity; Pesticide; Biosensing; METAL-ORGANIC-FRAMEWORK; PESTICIDE EXPOSURE; BIOSENSOR; WATER; PHOTOCATALYSTS; RESIDUES; DESIGN; SOIL; OPH;
D O I
10.1016/j.envres.2019.04.018
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The hexahistidine-tagged organophosphorus hydrolase (OPH6His) has been immobilized on a Zr-MOF, namely UiO-66-NH2. The resulting enzyme-MOF composite was used as a carrier to facilitate the hydrolysis of an organophosphate pesticide, i.e., methyl parathion in to p-nitrophenol (PNP). The formation of PNP took place in direct proportion to the added pesticide concentration. Coumarin1 (7-diethylamino-4-methylcoumarin) was then introduced in the reaction mixture as a reporter fluorescent molecule. As PNP acted to quench the fluorescence of coumarin1, it became possible to detect methyl parathion over a wide concentration range of 10-10(6) ng/mL with an achievable limit of quantification as 10 ng/mL. The immobilization of OPH6His on the surface of UiO-66-NH2 was found to endow an improvement in the enzymatic activity by about 37%. The OPH6(His)/UiO-66-NH2 conjugate was reusable for at least up to eight times and also found stable toward long-term storage (minimum 60 days). The potential practical utility of the above proposed sensing method has been demonstrated by employing it for an accurate analysis of pesticide-spiked food samples.
引用
收藏
页码:46 / 53
页数:8
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