DNA methylation of Quercus robur L. plumules following cryo-pretreatment and cryopreservation

被引：19

作者：

Plitta, Beata P. ^{[1
]}

Michalak, Marcin ^{[1
]}

Naskret-Barciszewska, Miroslawa Z. ^{[2
]}

Barciszewski, Jan ^{[2
]}

Chmielarz, Pawel ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Dendrol, Seed Biol Dept, Parkowa 5, PL-62035 Kornik, Poland

[2] Polish Acad Sci, Inst Bioorgan Chem, Dept RNA Biol, PL-61704 Poznan, Poland

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2014年 / 117卷 / 01期

关键词：

Cryopreservation; Pedunculate oak; Genetic resources; Liquid nitrogen; Desiccation; 5-Methylcytosine; GENETIC-RESOURCES; PATTERNS; CYTOSINE; DIFFERENTIATION; INTEGRITY; STORAGE; STRESS; CELLS;

D O I：

10.1007/s11240-013-0417-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pedunculate oak (Quercus robur) is an ecologically and economically important forest tree species which produces seeds that are classified as recalcitrant. Thus, cryopreservation of seed meristems is a method for long-term preservation of this germplasm in gene banks. During cryopreservation, many factors, such as desiccation, cryoprotection and cooling/rewarming, can induce stress in the frozen meristems. In this study, in vitro survival and the global DNA methylation level of plumules after cryoprotection, desiccation and cryostorage was evaluated. Results indicated that both desiccation and storage in liquid nitrogen have negligible influence on DNA methylation status of Q. robur plumules. These findings support the cryopreservation of plumules as an appropriate method for conservation of Q. robur germplasm.

引用

页码：31 / 37

页数：7

共 46 条

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