DNA methylation of Quercus robur L. plumules following cryo-pretreatment and cryopreservation

被引：19

作者：

Plitta, Beata P. ^{[1
]}

Michalak, Marcin ^{[1
]}

Naskret-Barciszewska, Miroslawa Z. ^{[2
]}

Barciszewski, Jan ^{[2
]}

Chmielarz, Pawel ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Dendrol, Seed Biol Dept, Parkowa 5, PL-62035 Kornik, Poland

[2] Polish Acad Sci, Inst Bioorgan Chem, Dept RNA Biol, PL-61704 Poznan, Poland

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2014年 / 117卷 / 01期

关键词：

Cryopreservation; Pedunculate oak; Genetic resources; Liquid nitrogen; Desiccation; 5-Methylcytosine; GENETIC-RESOURCES; PATTERNS; CYTOSINE; DIFFERENTIATION; INTEGRITY; STORAGE; STRESS; CELLS;

D O I：

10.1007/s11240-013-0417-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pedunculate oak (Quercus robur) is an ecologically and economically important forest tree species which produces seeds that are classified as recalcitrant. Thus, cryopreservation of seed meristems is a method for long-term preservation of this germplasm in gene banks. During cryopreservation, many factors, such as desiccation, cryoprotection and cooling/rewarming, can induce stress in the frozen meristems. In this study, in vitro survival and the global DNA methylation level of plumules after cryoprotection, desiccation and cryostorage was evaluated. Results indicated that both desiccation and storage in liquid nitrogen have negligible influence on DNA methylation status of Q. robur plumules. These findings support the cryopreservation of plumules as an appropriate method for conservation of Q. robur germplasm.

引用

页码：31 / 37

页数：7

共 46 条

[1] TLC-based detection of methylated cytosine: application to aging epigenetics
Barciszewska, Miroslawa Z.
Barciszewska, Anna Maria
Rattan, Suresh I. S.
[J]. BIOGERONTOLOGY, 2007, 8 (06) : 673 - 678
[2] DIFFERENTIATION OF 2 MOUSE-CELL LINES IS ASSOCIATED WITH HYPOMETHYLATION OF THEIR GENOMES
BESTOR, TH
HELLEWELL, SB
INGRAM, VM
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (09) : 1800 - 1806
[3] Blomme R, 1986, VERBONDSNIEUWS BELGI, V30, P771
[4] Climate influence on radial increment of oak (Quercus SP.) in central Poland
Bronisz, Agnieszka
Bijak, Szymon
Bronisz, Karol
Zasada, Michal
[J]. GEOCHRONOMETRIA, 2012, 39 (04): : 276 - 284
[5] The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos
Businge, Edward
Bygdell, Joakim
Wingsle, Gunnar
Moritz, Thomas
Egertsdotter, Ulrika
[J]. PHYSIOLOGIA PLANTARUM, 2013, 149 (02) : 273 - 285
[6] DNA methylating and demethylating treatments modify phenotype and cell wall differentiation state in sugarbeet cell lines
Causevic, A
Delaunay, A
Ounnar, S
Righezza, M
Delmotte, F
Brignolas, F
Hagège, D
Maury, S
[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2005, 43 (07) : 681 - 691
[7] Changes in methylation and structure of DNA from almond tissues during in vitro culture and cryopreservation
Channuntapipat, C
Sedgley, M
Collins, G
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2003, 128 (06) : 890 - 897
[8] Successful cryopreservation of Quercus robur plumules
Chmielarz, Pawel
Michalak, Marcin
Palucka, Malgorzata
Wasilenczyk, Urszula
[J]. PLANT CELL REPORTS, 2011, 30 (08) : 1405 - 1414
[9] DNA methylation analysis techniques
Dahl, C
Guldberg, P
[J]. BIOGERONTOLOGY, 2003, 4 (04) : 233 - 250
[10] THE EFFECT OF DRYING QUERCUS-ROBUR ACORNS TO DIFFERENT MOISTURE CONTENTS, FOLLOWED BY STORAGE, EITHER WITH OR WITHOUT IMBIBITION
GOSLING, PG
[J]. FORESTRY, 1989, 62 (01): : 41 - 50

← 1 2 3 4 5 →