Physical, chemical, and regulatory properties of glycolate oxidase in C3 and C4 plants

被引:2
作者
Eprintsev, A. T. [1 ]
Semenov, A. E. [1 ]
Navid, M. [1 ]
Popov, V. N. [1 ]
机构
[1] Voronezh State Univ, Dept Plant Physiol & Biochem, Fac Biol & Soil Sci, Voronezh 394006, Russia
基金
俄罗斯基础研究基金会;
关键词
Zea mays; Glycine max; glycolate oxidase; mesophyll; cortex; electrophoresis; gel chromatography; PURIFICATION; LEAVES;
D O I
10.1134/S1021443709020034
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Physical, chemical, and regulatory properties of glycolate oxidase (GO) isolated from the leaves of C-4 and C-3 plants (Zea mays L., cv. Voronezhskaya 76 and Glycine max (L.) Merr., cv. Pripyat', respectively) were studied. The homogenous preparations were obtained by multistage enzyme purification from soybean leaves and maize mesophyll and bundle sheath. The glycolate oxidase (GO) preparations obtained consisted of two types of subunits, 37 and 44 kD. The GO isolated from C-3 plant leaves had many in common with that extracted from C-4 plant bundle sheath as regards physical, chemical, and catalytic properties. The primary function of GO in both plant types is metabolism of glycolate, which is a product of ribulosebisphosphate oxalacetic acid oxidation and is used by plants for biosynthesis of hydrocarbons and amino acids.
引用
收藏
页码:164 / 167
页数:4
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