Determination of tryptoquialanines A and C produced by Penicillium digitatum in oranges: Are we safe?

被引：19

作者：

Araujo, Eder de Vilhena ^{[1
]}

Vendramini, Pedro Henrique ^{[1
]}

Costa, Jonas Henrique ^{[1
]}

Eberlin, Marcos Nogueira ^{[1
]}

Montagner, Cassiana Carolina ^{[1
]}

Fill, Taicia Pacheco ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP, Brazil

来源：

FOOD CHEMISTRY | 2019年 / 301卷

基金：

巴西圣保罗研究基金会;

关键词：

Tryptoquialanines; Penicillium digitatum; QuEChERS; Mycotoxins; ALTERNARIOL MONOMETHYL; MASS-SPECTROMETRY; CITRUS; VERRUCULOGEN; EXTRACTION; TISSUES; ETHER;

D O I：

10.1016/j.foodchem.2019.125285

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method was developed and validated for determination of tryptoquialanines A and C in orange samples on epicarp (exterior peel), mesocarp (white peel), and endocarp (fruit juice) based on QuEChERS extraction and LC-MS/MS analysis. The method showed an excellent linearity over a range of 5-400 mu g kg(-1), with r(2) >= 0.998. The limits of detection (LOD) and quantification (LOQ) were 5 and 10 mu g kg(-1), respectively. Recoveries showed values between 57 and 101%, with RSD <= 12%. Analysis of infected oranges showed diffusion of the alkaloids between the orange layers after 4 days post infection in concentrations > LOQ. Mycotoxin diffusion to healthy oranges after direct contact with infected oranges for 48 h, showed alkaloid concentrations >= 10 mu g kg(-1) on epicarp layer. The developed method can be easily applied for quality control in routine analysis of orange fruit due to the high risk that these tremorgenic alkaloids represent to human health.

引用

页数：6

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