Development of a Powder Extruder System for Dual-pore Tissue-engineering Scaffold Fabrication

被引:8
|
作者
Kang, Nae-Un [1 ]
Hong, Myoung Wha [2 ]
Kim, Young Yul [2 ]
Cho, Young-Sam [1 ]
Lee, Seung-Jae [1 ]
机构
[1] Wonkwang Univ, Coll Engn, Dept Mech Design Engn, 460 Iksandae Ro, Iksan Jeonbuk 570749, South Korea
[2] Catholic Univ Korea, Daejeon St Marys Hosp, Dept Orthoped, 64 Daeheung Ro, Daejeon 301723, South Korea
关键词
dual-pore scaffold; powder extruder system; polycaprolactone; 3D tissue-engineering scaffold; BIODEGRADABLE POLYMER SCAFFOLDS; POROUS SCAFFOLDS; POLY(D; L-LACTIC-CO-GLYCOLIC ACID); DEGRADATION BEHAVIOR; GELATIN SCAFFOLDS; SALT; BONE; CARTILAGE; SPONGES;
D O I
10.1007/s42235-019-0055-y
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
In this study, we developed a powder extruder system that can extrude and deposit powder mixtures to overcome the reported limitations of conventional dual-pore scaffold manufacturing methods. To evaluate the extrusion and deposition capability of the powder extruder system, 3D tissue-engineering scaffolds with dual-pore characteristics were fabricated with a PCL/PEO/NaCl (polycaprolactone/polyethylene oxide/sodium chloride) powder mixture. In addition, to evaluate the fabricated scaffolds, their compressive modulus, morphology, and in-vitro cell activity were assessed. Consequently, it was confirmed that the proposed powder extruder system can fabricate dual-pore scaffolds with well-interconnected pores as well as arbitrary 3D shapes shown by the fabrication of a 3D femur-shape scaffold similar to the femur model. The results of the cell proliferation and Cell Counting Kit-8 (CCK-8) assays, DNA content analysis and viability assays confirm that the dual-pore scaffold fabricated by the powder extruder system improves cell attachment, proliferation, and viability.
引用
收藏
页码:686 / 695
页数:10
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