Adhesion and transcellular migration of neutrophils and B lymphocytes on fibroblasts

被引:21
作者
Couture, Patrick [1 ]
Paradis-Massie, Jeremie [1 ]
Oualha, Nadia [1 ]
Thibault, Gaetan [1 ]
机构
[1] Univ Montreal, Inst Rech Clin Montreal, Montreal, PQ H2W 1R7, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
Cell trafficking; Emperipolesis; B cells; Neutrophils; Adhesion molecules; Fibroblasts; ROSAI-DORFMAN-DISEASE; STROMAL CELLS; MATRIX INTERACTIONS; LUNG FIBROBLASTS; SYNOVIAL TISSUE; LYMPHOID-CELLS; IMMUNE-SYSTEM; RAT LEUKOCYTE; BETA(2) CD18; BONE-MARROW;
D O I
10.1016/j.yexcr.2009.04.013
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
During tissue inflammation, infiltrated leukocytes may have physical contacts with fibroblasts. We observed that neutrophils and B lymphocytes adhered in a larger proportion than T cells on cultured fibroblasts. Microscopy showed that adhesion was also characterized by leukocyte engulfment by the fibroblasts. In migration assays, only neutrophils and B lymphocytes were selectively able to migrate through a fibroblast barrier. Adhesion and migration were increased by stimulation with tumor necrosis factor-alpha (TNF-alpha) and phorbol-12-myristate-13-acetate (PMA). Antibodies against ICAM-1/beta 2 integrin blocked the interaction of neutrophils to fibroblasts. For B lymphocytes the couple VCAM-1/alpha 4 integrin was also involved in this interaction. Human skin fibroblasts presented similar adhesion characteristics as rat cardiac fibroblasts. By measuring the distance between the border of migration holes and cadherin-positive adherens junctions, more than 65% of the holes correspond to the transcellular route over the paracellular route. Furthermore, vimentin staining revealed that the migration holes were highly nested by intermediate filaments in accordance with the transcellular route. Our results demonstrated that engulfment of neutrophils and B lymphocytes by. fibroblasts resulted in selective passage by a transcellular route. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:2192 / 2206
页数:15
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