Separation of Phenylpropanoid Glycosides from a Chinese Herb by HSCCC

被引：17

作者：

Chen, Chen ^{[1
]}

Zhao, Xiao-Hui ^{[1
,2
,3
]}

Yue, Hui-Lan ^{[1
]}

Li, Yu-Lin ^{[1
]}

Chen, Tao ^{[1
]}

机构：

[1] Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China

[2] Key Lab Plateau Crop Germplasm Innovat & Utilizat, State Key Lab Breeding Base, Xining 810016, Peoples R China

[3] Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2014年 / 52卷 / 05期

关键词：

PHENYLETHANOID GLYCOSIDES; PURIFICATION; ACTEOSIDE; ISOACTEOSIDE; EXTRACTS;

D O I：

10.1093/chromsci/bmt048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenyl-propanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 mg/mL, respectively.

引用

页码：395 / 399

页数：5

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