Degradation and interconversion of plant pteridines during sample preparation and ultra-high performance liquid chromatography-tandem mass spectrometry

被引：6

作者：

Van Daele, Jeroen ^{[1
]}

Blancquaert, Dieter ^{[2
]}

Kiekens, Filip ^{[1
]}

Van Der Straeten, Dominique ^{[2
]}

Lambert, Willy E. ^{[1
]}

Stove, Christophe P. ^{[1
]}

机构：

[1] Univ Ghent, Dept Bioanal, Lab Toxicol, B-9000 Ghent, Belgium

[2] Univ Ghent, Dept Physiol, Lab Funct Plant Biol, B-9000 Ghent, Belgium

来源：

FOOD CHEMISTRY | 2016年 / 194卷

关键词：

(U)HPLC-MS/MS; Pterins; Stability; Degradation; Interconversion; Potato; Arabidopsis; ALKALINE AQUEOUS-SOLUTIONS; FOLATE BIOSYNTHESIS GENES; URINE SPECIFIC-GRAVITY; EARLY CANCER-DETECTION; ARABIDOPSIS-THALIANA; POTATO-TUBERS; FOLIC-ACID; PTERIN; RICE; HPLC;

D O I：

10.1016/j.foodchem.2015.08.098

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The degradation and interconversion of a selected set of pterins (dihydroneopterin, hydroxymethyldihydropterin, diydroxanthopterin, neopterin, hydroxymethylpterin, xanthopterin, 6-formylpterin, 6-carboxypterin and pterin), spiked to charcoal-treated potato and Arabidopsis thaliana matrix was investigated, together with their relative recovery in potato and A. thaliana. As a result, a matrix-specific procedure for the ultra-high performance liquid chromatography-tandem mass spectrometry based determination of 6 aromatic pterins (neopterin, hydroxymethylpterin, xanthopterin, 6-formylpterin, 6-carboxypterin and pterin) is proposed: 1.5 ml of an N-2-flushed, alkaline (pH = 10) extraction solvent is added to 200 mg of plant sample. After boiling and homogenization, the samples are incubated: Arabidopsis samples for 30 min at room temperature, while shaking, and potato samples for 2 h at 37 degrees C (applying a dienzyme treatment with alpha-amylase and protease). After a final boiling step, the samples are ultrafiltrated and resulting extracts are analyzed by UHPLC-MS/MS. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1189 / 1198

页数：10

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