Anti adipogenic activity of Aegle marmelos Correa

In continuation of evaluating the anti-obesity effect of Aegle marmelos, we have screened the n-hexane, dichloro methane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts of the leaves at the concentration of 25, 50, 75 and 100 mu g/ml for adipogenesis inhibition in the adipocytes. Nile red staining with the help of fluorometry was used as indicator of the antiobesity activity.. The most active DCM extract showed the 33.98 +/- 3.55% lipid content at 100 mu g/ml and was selected for the further isolation. 14 compounds were isolated from DCM extract of A. marmelos leaves. The compounds were screened for the adipogenesis inhibition at 50 and 100 mu M concentrations. Out of the 14 compounds, halfordinol, ethyl ether aegeline and esculetin were showing 10.04 +/- 0.52, 16.29 +/- 0.85 and 25.09 +/- 1.31% lipid content respectively at 100 mu M. We hereby report the adipogenesis inhibition by A. marmelos as one of the pathway for its antiobesity effect. (C) 2013 Elsevier GmbH. All rights reserved.