Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers

被引：0

作者：

Moore, D ^{[1
]}

Michael, H ^{[1
]}

Tritt, R ^{[1
]}

Kelley, M ^{[1
]}

机构：

[1] Indiana Univ, Sch Med, Dept Obstet & Gynecol, Indianapolis, IN 46204 USA

来源：

7TH BIENNIAL MEETING OF THE INTERNATIONAL GYNECOLOGIC CANCER SOCIETY | 1999年

关键词：

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The DNA base excision repair (BER) pathway is responsible for the repair of cellular alkylation and oxidative DNA damage. A crucial step in the BER pathway involves the cleavage of an apurinic/apyrimidinic site in DNA by an AP endonuclease. The major AP endonuclease in mammalian cells is APE/ref-1. We studied the expression of APE/ref-1 in formalin-fixed, paraffin-embedded ovarian tissue specimens using polyclonal and monoclonal antibodies to APE/ref-1. Compared to normal ovarian tissues, benign neoplastic conditions, and low malignant potential tumors, APE/ref-1 immunoreactivity is altered in malignant ovarian tumors. Further studies will determine if the altered expression reflects changes in DNA repair capabilities or redox regulatory functions.

引用

页码：263 / 267

页数：5

共 11 条

[1] Identification of redox/repair protein Ref-1 as a potent activator of p53
Jayaraman, L
Murthy, KGK
Zhu, C
Curran, T
Xanthoudakis, S
Prives, C
[J]. GENES & DEVELOPMENT, 1997, 11 (05) : 558 - 570
[2] KELLEY MR, 1998, GERM CELL TUMORS
[3] MUTAGENESIS BY APURINIC APYRIMIDINIC SITES
LOEB, LA
PRESTON, BD
[J]. ANNUAL REVIEW OF GENETICS, 1986, 20 : 201 - 230
[4] Genetic interaction between HAP1/REF-1 and p53
Meira, LB
Cheo, DL
Hammer, RE
Burns, DK
Reis, A
Friedberg, EC
[J]. NATURE GENETICS, 1997, 17 (02) : 145 - 145
[5] Redox state regulates binding of p53 to sequence-specific DNA, but not to non-specific or mismatched DNA
Parks, D
Bolinger, R
Mann, K
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (06) : 1289 - 1295
[6] Activation of apurinic/apyrimidinic endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxicity of free radicals
Ramana, CV
Boldogh, I
Izumi, T
Mitra, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (09) : 5061 - 5066
[7] Robertson KA, 1997, CELL GROWTH DIFFER, V8, P443
[8] Pyrrolidine dithiocarbamate prevents p53 activation and promotes p53 cysteine residue oxidation
Wu, HH
Momand, J
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (30) : 18898 - 18905
[9] REDOX ACTIVATION OF FOS JUN DNA-BINDING ACTIVITY IS MEDIATED BY A DNA-REPAIR ENZYME
XANTHOUDAKIS, S
MIAO, G
WANG, F
PAN, YCE
CURRAN, T
[J]. EMBO JOURNAL, 1992, 11 (09) : 3323 - 3335
[10] THE REDOX AND DNA-REPAIR ACTIVITIES OF REF-1 ARE ENCODED BY NONOVERLAPPING DOMAINS
XANTHOUDAKIS, S
MIAO, GG
CURRAN, T
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (01) : 23 - 27

← 1 2 →