A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis

被引：17

作者：

Harinath, Bhaskar Chinnaiah ^{[1
]}

Kumar, Satish

Roy, Santa Saha

Hirudkar, Suvarna

Upadhye, Vijay

Shende, Niraj

机构：

[1] Mahatma Gandhi Inst Med Sci, Jamnalal Bajaj Trop Dis Res Ctr, Wardha 442102, Maharashtra, India

[2] Mahatma Gandhi Inst Med Sci, Dept Biochem, Wardha 442102, Maharashtra, India

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2006年 / 55卷 / 01期

关键词：

mycobacterial ES antigen; ELISA; cocktail antibody; immune-complexed antigen; tuberculosis;

D O I：

10.1016/j.diagmicrobio.2005.10.021

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A cocktail of affinity-purified antibodies against diagnostically useful Mycobacterium tubereulosis H37Ra excretory-secretory protein antigens ES-31, ES-43, and EST-6 was explored for detection of circulating free and immune-complexed (IC) antigen in sera of patients with confirmed tuberculosis (TB) by sandwich enzyme-linked immunosorbent assay and compared with monospecific anti-ES-31 antibody. Out of 68 smear-positive TB cases studied, using cocktail antibody, a sensitivity of 97% (66/68) for immune-complexed cocktail antigen and 91% (62/68) for free cocktail-antigen detection was observed, compared to 91% (62/68) for immune-complexed ES-31 and 79% (54/68) for free ES-31 antigen when anti-ES-31 antibody was used alone. Thus, combinatorial use of antibodies showed improved sensitivity and was thus observed to be better than single antibody. The specificity was observed to be 99% for immune-complexed antigen using cocktail antibody. Furthermore, analysis of different groups of TB sera showed that circulating immune-complexed antigen is a sensitive marker than free antigen. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：65 / 68

页数：4

共 29 条

[1]

ASTEKAR MD, 1987, J CLIN LAB IMMUNOL, V45, P29

[2]

Banerjee S, 2003, INT J TUBERC LUNG D, V7, P278

[3]

Banerjee Swati, 2003, Indian J Pathol Microbiol, V46, P261

[4] CLINICAL-VALUE OF THE MEASUREMENT OF MYCOBACTERIUM-TUBERCULOSIS SPECIFIC ANTIBODY IN PULMONARY TUBERCULOSIS [J].

BOTHAMLEY, GH ;

RUDD, R ;

FESTENSTEIN, F ;

IVANYI, J .

THORAX, 1992, 47 (04) :270-275

[5] IMMUNE-COMPLEXES IN THE SPECTRUM OF TUBERCULOSIS [J].

BROSTOFF, J ;

LENZINI, L ;

ROTTOLI, P ;

ROTTOLI, L .

TUBERCLE, 1981, 62 (03) :169-173

[6] DETECTION OF MYCOBACTERIAL ANTIGEN AND ANTIBODIES IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH TUBERCULOUS MENINGITIS [J].

CHANDRAMUKI, A ;

ALLEN, PRJ ;

KEEN, M ;

IVANYI, J .

JOURNAL OF MEDICAL MICROBIOLOGY, 1985, 20 (02) :239-247

[7]

GUPTA S, 2005, CURR SCI, V88

[8]

GUPTA S, 2004, BIOMED RES, V15, P76

[9] Use of multiepitope polyproteins in serodiagnosis of active tuberculosis [J].

Houghton, RL ;

Lodes, MJ ;

Dillon, DC ;

Reynolds, LD ;

Day, CH ;

McNeill, PD ;

Hendrickson, RC ;

Skeiky, YAW ;

Sampaio, DP ;

Badaro, R ;

Lyashchenko, KP ;

Reed, SG .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2002, 9 (04) :883-891

[10] CIRCULATING IMMUNE-COMPLEXES IN TUBERCULOSIS [J].

JOHNSON, NM ;

MCNICOL, MW ;

BURTONKEE, EJ ;

MOWBRAY, JF .

THORAX, 1981, 36 (08) :610-617

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