Label-free selective sensing of Pb2+ lead(II) sensors based on the aggregation of a pyrene fluorescent probe

被引:8
|
作者
Yang, Yue [1 ,2 ]
Wang, Shujie [1 ]
Hu, Xinyu [1 ]
Yang, Xiangyu [2 ]
机构
[1] Jilin Univ, Coll Biol & Agr Engn, Changchun 130022, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
来源
CHINESE SCIENCE BULLETIN | 2014年 / 59卷 / 5-6期
基金
中国国家自然科学基金;
关键词
Pyrene probe; G-quadruplex; Pb2+ lead(II) ion; Label free; Fluorescence detection; EXONUCLEASE III; DNAZYME; ASSAY; IONS;
D O I
10.1007/s11434-013-0080-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the present work, we report a label-free fluorescence turn-on approach for the sensitive and selective sensing of Pb2+. Pyrene with one positive charge was used as the fluorescent probe, and thrombin aptamer (TBA), which was a G-rich oligonucleotide, was employed to form G-quadruplex with lead(II). When TBA and Pb2+ were mixed with lead(II) in an aqueous solution, it was folded into a stable G-quadruplex. Subsequently, a single-stranded nucleic acid-specific nuclease S1 was added. The G-quadruplex stabilized by Pb2+ lead(II) had markedly a significant resistant ability to nuclease S1 digestion. However, in the absence of Pb2+ lead(II), no quadruplex or less stable quadruplex was formed and TBA was digested by nuclease S1 in 3 min under the optimized experimental conditions. Finally, pyrene probe was mixed with oligonucleotide in Pb2+ lead(II). Electrostatic interactions between oligonucleotide (a polyanion) and the probe induced the aggregation of the probe, which in turn produced strong emission of the strong pyrene excimer emission. The intensity of the induced excimer emission was directly proportional to the amount of Pb2+ added. Our approach shows good selectivity and sensitivity for the detection of Pb2+ with a limit of detection limit as low as 800 nmol/L.
引用
收藏
页码:502 / 508
页数:7
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