Combination of Loop-Mediated Isothermal Amplification and AuNP-Oligoprobe Colourimetric Assay for Pork Authentication in Processed Meat Products

被引:12
作者
Thangsunan, Pattanapong [1 ]
Temisak, Sasithon [1 ]
Morris, Phattaraporn [1 ]
Rios-Solis, Leonardo [2 ,3 ]
Suree, Nuttee [4 ,5 ]
机构
[1] Natl Inst Metrol, Chem Metrol & Biometry Dept, Bioanal Grp, Pathum Thani, Thailand
[2] Univ Edinburgh, Inst Bioengn, Sch Engn, Edinburgh, Midlothian, Scotland
[3] Univ Edinburgh, Ctr Synthet & Syst Biol SynthSys, Edinburgh, Midlothian, Scotland
[4] Chiang Mai Univ, Div Biochem & Biochem Technol, Dept Chem, Fac Sci, Chiang Mai, Thailand
[5] Chiang Mai Univ, Ctr Excellence Mat Sci & Technol, Fac Sci, Chiang Mai, Thailand
关键词
Pork authentication; Pork adulteration; Halal food authentication; Loop-mediated isothermal amplification; AuNP-nanoprobe colourimetric assay; REAL-TIME PCR; FUNCTIONALIZED GOLD NANOPARTICLES; VISUAL DETECTION; DNA; IDENTIFICATION; LAMP; ADULTERATION; GENE; QUANTIFICATION; STABILIZATION;
D O I
10.1007/s12161-020-01901-5
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Pork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well as those who are allergic to pork meat and its derivatives. Accurate pork authentication is of great importance to assist this demographic group of people in making decision on their product purchase. The aim of this study was to develop a new analytical method for pork authentication in processed meat products based on a combination of loop-mediated isothermal amplification (LAMP) and AuNP-nanoprobe colourimetric assay. The LAMP conditions were first optimised to obtain the highest yield of amplified DNA products within the shortest time. Oligoprobe-functionalised AuNPs were then hybridised with LAMP-DNA amplicons and subsequently challenged with MgSO4 at a high concentration to induce AuNP aggregation. In the presence of pork DNA, the colloidal AuNP-probe remained unchanged in its red colour, which indicates the dispersion of AuNPs. In contrast, in the absence of pork DNA, the colour was changed to colourless as a result from the aggregation of AuNPs. The LAMP-AuNP-nanoprobe assay offers a high sensitivity with a limit of detection as low as 100 pg of pork DNA. The assay is highly specific to pork content without cross-reactivity with the other meat species tested. The assay developed herein can become a simple, inexpensive, precise, and rapid analytical tool for small laboratories or the general public interested in halal food authentication.
引用
收藏
页码:568 / 580
页数:13
相关论文
共 74 条
  • [1] Gold nanoparticle-based colorimetric biosensors
    Aldewachi, H.
    Chalati, T.
    Woodroofe, M. N.
    Bricklebank, N.
    Sharrack, B.
    Gardiner, P.
    [J]. NANOSCALE, 2018, 10 (01) : 18 - 33
  • [2] Gold Nanoparticle Sensor for the Visual Detection of Pork Adulteration in Meatball Formulation
    Ali, M. E.
    Hashim, U.
    Mustafa, S.
    Man, Y. B. Che
    Islam, Kh N.
    [J]. JOURNAL OF NANOMATERIALS, 2012, 2012
  • [3] Species identification and animal authentication in meat products: a review
    Alikord, Mahsa
    Momtaz, Hassan
    Keramat, Javad
    Kadivar, Mahdi
    Rad, Aziz Homayouni
    [J]. JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, 2018, 12 (01) : 145 - 155
  • [4] [Anonymous], 2018, Livestock and Poultry: World Markets and Trade
  • [5] [Anonymous], 2019, MEAT MARK REV OV GLO
  • [6] Authentication of meat and meat products
    Ballin, N. Z.
    [J]. MEAT SCIENCE, 2010, 86 (03) : 577 - 587
  • [7] Species determination - Can we detect and quantify meat adulteration?
    Ballin, Nicolai Z.
    Vogensen, Finn K.
    Karlsson, Anders H.
    [J]. MEAT SCIENCE, 2009, 83 (02) : 165 - 174
  • [8] Detection of pork adulteration in processed meat by species-specific PCR-QIAxcel procedure based on D-loop and cytb genes
    Barakat, Hassan
    El-Garhy, Hoda A. S.
    Moustafa, Mahmoud M. A.
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (23) : 9805 - 9816
  • [9] Digital Assays Part I: Partitioning Statistics and Digital PCR
    Basu, Amar S.
    [J]. SLAS TECHNOLOGY, 2017, 22 (04): : 369 - 386
  • [10] Loop-mediated isothermal amplification (LAMP) - review and classification of methods for sequence-specific detection
    Becherer, Lisa
    Borst, Nadine
    Bakheit, Mohammed
    Frischmann, Sieghard
    Zengerle, Roland
    von Stetten, Felix
    [J]. ANALYTICAL METHODS, 2020, 12 (06) : 717 - 746