Pork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well as those who are allergic to pork meat and its derivatives. Accurate pork authentication is of great importance to assist this demographic group of people in making decision on their product purchase. The aim of this study was to develop a new analytical method for pork authentication in processed meat products based on a combination of loop-mediated isothermal amplification (LAMP) and AuNP-nanoprobe colourimetric assay. The LAMP conditions were first optimised to obtain the highest yield of amplified DNA products within the shortest time. Oligoprobe-functionalised AuNPs were then hybridised with LAMP-DNA amplicons and subsequently challenged with MgSO4 at a high concentration to induce AuNP aggregation. In the presence of pork DNA, the colloidal AuNP-probe remained unchanged in its red colour, which indicates the dispersion of AuNPs. In contrast, in the absence of pork DNA, the colour was changed to colourless as a result from the aggregation of AuNPs. The LAMP-AuNP-nanoprobe assay offers a high sensitivity with a limit of detection as low as 100 pg of pork DNA. The assay is highly specific to pork content without cross-reactivity with the other meat species tested. The assay developed herein can become a simple, inexpensive, precise, and rapid analytical tool for small laboratories or the general public interested in halal food authentication.
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Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Univ Mosul, Pharm Coll, Mosul, IraqSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Aldewachi, H.
Chalati, T.
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Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Univ Lincoln, Sch Pharm, Lincoln, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Chalati, T.
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Woodroofe, M. N.
Bricklebank, N.
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Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Bricklebank, N.
Sharrack, B.
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Univ Sheffield, Dept Neurol, Sheffield, S Yorkshire, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Sharrack, B.
Gardiner, P.
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Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
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Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Perlis, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Ali, M. E.
Hashim, U.
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Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Perlis, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Hashim, U.
Mustafa, S.
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Univ Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Mustafa, S.
Man, Y. B. Che
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Univ Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Man, Y. B. Che
Islam, Kh N.
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Univ Putra Malaysia, Dept Preclin Sci, Fac Vet Med, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
机构:
Tabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Alikord, Mahsa
Momtaz, Hassan
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机构:
Islamic Azad Univ, Fac Vet Med, Dept Microbiol, Shahrekord Branch, Shahrekord, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Momtaz, Hassan
Keramat, Javad
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Isfahan Univ Technol, Fac Agr, Dept Food Sci & Technol, Esfahan, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Keramat, Javad
Kadivar, Mahdi
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Isfahan Univ Technol, Fac Agr, Dept Food Sci & Technol, Esfahan, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Kadivar, Mahdi
Rad, Aziz Homayouni
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Tabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
机构:
Wayne State Univ, Dept Elect & Comp Engn, Detroit, MI 48202 USA
Wayne State Univ, Dept Biomed Engn, Detroit, MI USAWayne State Univ, Dept Elect & Comp Engn, Detroit, MI 48202 USA
机构:
Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Univ Mosul, Pharm Coll, Mosul, IraqSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Aldewachi, H.
Chalati, T.
论文数: 0引用数: 0
h-index: 0
机构:
Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Univ Lincoln, Sch Pharm, Lincoln, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Chalati, T.
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Woodroofe, M. N.
Bricklebank, N.
论文数: 0引用数: 0
h-index: 0
机构:
Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Bricklebank, N.
Sharrack, B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sheffield, Dept Neurol, Sheffield, S Yorkshire, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
Sharrack, B.
Gardiner, P.
论文数: 0引用数: 0
h-index: 0
机构:
Sheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, EnglandSheffield Hallam Univ, Biomol Sci Res Ctr, Sheffield, S Yorkshire, England
机构:
Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Perlis, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Ali, M. E.
Hashim, U.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Perlis, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Hashim, U.
Mustafa, S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Mustafa, S.
Man, Y. B. Che
论文数: 0引用数: 0
h-index: 0
机构:
Univ Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
Man, Y. B. Che
Islam, Kh N.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Putra Malaysia, Dept Preclin Sci, Fac Vet Med, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia
机构:
Tabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Alikord, Mahsa
Momtaz, Hassan
论文数: 0引用数: 0
h-index: 0
机构:
Islamic Azad Univ, Fac Vet Med, Dept Microbiol, Shahrekord Branch, Shahrekord, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Momtaz, Hassan
Keramat, Javad
论文数: 0引用数: 0
h-index: 0
机构:
Isfahan Univ Technol, Fac Agr, Dept Food Sci & Technol, Esfahan, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Keramat, Javad
Kadivar, Mahdi
论文数: 0引用数: 0
h-index: 0
机构:
Isfahan Univ Technol, Fac Agr, Dept Food Sci & Technol, Esfahan, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
Kadivar, Mahdi
Rad, Aziz Homayouni
论文数: 0引用数: 0
h-index: 0
机构:
Tabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, IranTabriz Univ Med Sci, Fac Nutr, Dept Food Sci & Technol, Tabriz, Iran
机构:
Wayne State Univ, Dept Elect & Comp Engn, Detroit, MI 48202 USA
Wayne State Univ, Dept Biomed Engn, Detroit, MI USAWayne State Univ, Dept Elect & Comp Engn, Detroit, MI 48202 USA