Ultrastructural Study of Neuronal Death in Rat Hippocampus after Transient and Permanent Focal Cerebral Ischemia

被引:0
作者
Asadi-Shekalari, Majid [1 ]
Panahi, Marzieh [2 ]
Vaghefi, Seyed Hassan Eftekhar [3 ]
Zadeh, Ali Aboli Dokht Noughi [3 ]
机构
[1] Kerman Neurosci Res Ctr KNRC, Dept Electron Microscopy, Kerman, Iran
[2] Ahwaz Jondi Shapour Med Univ, Sch Med, Dept Histol & Embryol, Ahvaz, Iran
[3] Kerman Med Univ, Sch Med, Dept Anat, Kerman, Iran
来源
YAKHTEH | 2009年 / 11卷 / 01期
关键词
Cell Death; Rat; Hippocampus; Ischemia; ARTERY OCCLUSION; FOREBRAIN ISCHEMIA; APOPTOSIS; NECROSIS; MECHANISMS;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objective: Morphological changes of CA1 neurons in rat hippocampus after transient and permanent focal cerebral ischemia were studied to clarify the nature of post-ischemic cell death in the subfield. Materials and Methods: Male adult rats were divided into 3 groups: Control (Sham-operated), transient ischemic group (30 minutes of MCAO followed by 48 hours of reperfusion), and permanent ischemic group (48 hours of MCAO). After the mentioned times, deep anesthesia was induced in the rats and their brains were removed and processed for transmission electron microscopy (TEM) and evaluation. Results: Electron-microscopic examination on day 2 showed key morphological signs of apoptosis in the permanent ischemic group, while morphological signs of necrosis were observed in the transient ischemic group. Conclusion: These results suggest necrosis (as dominant mechanism of neuronal death after transient ischemia) and apoptosis (after permanent ischemia) to be involved in neuronal death.
引用
收藏
页码:23 / +
页数:7
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