Immobilization of horseradish peroxidase in phospholipid-templated titania and its applications in phenolic compounds and dye removal

被引：49

作者：

Jiang, Yanjun ^{[1
]}

Tang, Wei ^{[1
]}

Gao, Jing ^{[1
]}

Zhou, Liya ^{[1
]}

He, Ying ^{[1
]}

机构：

[1] Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2014年 / 55卷

关键词：

Horseradish peroxidase; Phospholipid-templated titania; Enzyme encapsulation; Phenolic compound; Dye; SOL-GEL MATRIX; ENZYME IMMOBILIZATION; BIOCOMPATIBLE METHOD; MESOPOROUS TITANIA; ENCAPSULATION; SILICA; NANOPARTICLES; DEGRADATION; STABILITY; MICROSPHERES;

D O I：

10.1016/j.enzmictec.2013.11.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, horseradish peroxidase (HRP) was encapsulated in phospholipid-templated titania particles through the biomimetic titanification process and used for the treatment of wastewater polluted with phenolic compounds and dye. The encapsulated HRP exhibited improved thermal stability, a wide range of pH stability and high tolerance against inactivating agents. It was observed an increase in K-m value for the encapsulated HRP (8.21 mM) when compared with its free counterpart. For practical applications in the removal of phenolic compounds and dye by the encapsulated HRP, the removal efficiency for phenol, 2-chlorophenol, Direct Black-38 were 92.99%, 87.97%, and 79.72%, respectively, in the first treatment cycle. Additionally, the encapsulated HRP showed better removal efficiency than free HRP and a moderately good capability of reutilization. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：1 / 6

页数：6

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