Cationic micelle based vaccine induced potent humoral immune response through enhancing antigen uptake and formation of germinal center

被引：22

作者：

Luo, Zichao ^{[1
,2
]}

Shi, Shuai ^{[3
,4
]}

Jin, Ling ^{[3
,4
]}

Xu, Lu ^{[3
,4
]}

Yu, Jing ^{[1
,2
]}

Chen, Hao ^{[3
,4
]}

Li, Xingyi ^{[3
,4
]}

机构：

[1] Wenzhou Med Univ, Inst Biomat & Engn, Wenzhou 325035, Peoples R China

[2] Wenzhou Inst Biomat & Engn, Wenzhou 325035, Peoples R China

[3] Wenzhou Med Univ, Sch Ophthalmol & Optometry, Inst Biomed Engn, Wenzhou 325027, Peoples R China

[4] Wenzhou Med Univ, Hosp Eye, Wenzhou 325027, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 135卷

基金：

中国国家自然科学基金;

关键词：

Micelle-based vaccine; Adjuvant; Immune response; Germinal center; G-PEI MICELLES; DELIVERY; CELLS; NANOPARTICLES; ISOTYPE; SYSTEM; GENE; NEED; SIZE;

D O I：

10.1016/j.colsurfb.2015.07.079

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Nanoparticles have been proven to be an effective vaccine delivery system that can boost immune responses to subunit vaccines. Herein, we developed and characterized a cationic polymeric polyethylene glycol(2000)-poly epsilon-caprolactone(2000)-polyethylenimine(2000) (mPEG(2000)-PCL2000-g-PEI2000) micelle as a potent vaccine delivery system to boost the immune response in vivo. The micelles that we developed exhibited great antigen-loading capability and minimal cytotoxicity in vitro. Meanwhile, micelles facilitated OVA antigen uptake by dendritic cells both in vitro and in vivo. More importantly, a micelle-formulated OVA vaccine could significantly promote anti-OVA antibody production by 190-fold and potently enhance T cell proliferation and the secretion of IL-5 and IFN-gamma. We attributed these effects to its ability to promote antigen uptake, antigen deposition, and germinal center formation. In conclusion, the mPEG(2000)-PCL2000-PEI2000 micelle that we developed has potential as potent vaccine delivery system to induce Th2 immune response. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：556 / 564

页数：9

共 39 条

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