Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor

被引：59

作者：

Kanno, K

Maeda, H

Izumo, S

Ikuno, M

Takeshita, K

Tashiro, A

Fujii, M

机构：

[1] Kinki Univ, Kyushu Sch Engn, Dept Bioenvironm Chem, Iizuka, Fukuoka 8208555, Japan

[2] AIST, Tosu, Saga 8410052, Japan

[3] Ctr Adv Technol, HKL, Iizuka, Fukuoka 8208555, Japan

来源：

LAB ON A CHIP | 2002年 / 2卷 / 01期

关键词：

D O I：

10.1039/b108741j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosidase-promoted hydrolysis was performed in a microreaction channel. The result was compared with the reaction in a micro-test tube. Transgalactosylation on p-nitrophenyl-2-acetamide-2-deoxy-beta-D-glucopyranoside was also examined in a microreaction channel, because transglycosylation is a useful method for oligosaccharide synthesis. We examined both the forward reaction, i.e., hydrolysis, and the reverse reaction, i.e., transglycosylation, in the microreaction channel. The results showed that both hydrolysis and transglycosylation were enhanced in the microreaction channel compared with the batch reaction.

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页码：15 / 18

页数：4

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