Cell to cell interaction between mesangial cells and macrophages induces the expression of monocyte chemoattractant protein-1 through nuclear factor-κB activation

The interaction between mesangial cells (MCs) and monocytes/macrophages (Mo/Mo) is an important pathogenic feature of glomerulonephritis. However, its mechanism is not fully elucidated. Studies to date have focused on the interactions through mediators. In the present study, to obtain insight into the mechanism of the interaction between MCs and Mo/Mo, we examined the significance of the cell to cell interaction of these cells in the context of monocyte chemoattractant protein-1 (MCP-1) expression using cell contact cultures or co-culture without contact. Our results revealed that the cellular adhesion of cultured macrophages to MCs induced the expression of MCP-1, which was mainly observed in the MCs. In addition, the induction of MCP-I was, at least in part, mediated by nuclear factor kappa-B activation which occurs preferentially in the MCs. Because MCP-1 is suggested to play an important role in glomerulonephritis, this novel cell to cell interaction between the MCs and Mo/Mo could be important in glomerulonephritis. (C) 2000 Academic Press.