Performance of the NG OligoGen kit for the diagnosis of Neisseria gonorrhoeae: comparison with cobas 4800 assay

被引：0
|
作者：
Parra-Sanchez, M. [1 ]
Garcia-Rey, S. [1 ]
Marcuello, A. [2 ]
Zakariya-Yousef, I. [1 ]
Bernal, S. [1 ]
Pueyo, I. [3 ]
Martin-Mazuelos, E. [1 ]
Palomares, J. C. [1 ]
机构：
[1] Valme Univ Hosp, Unit Infect Dis & Clin Microbiol, Seville, Spain
[2] Operon Inmuno & Mol Diagnost, Zaragoza, Spain
[3] Ctr Sexually Transmitted Infect Seville, Seville, Spain
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2016年 / 84卷 / 01期
关键词：
Sexually transmitted infections; Neisseria gonorrhoeae; Urine; Diagnostic test; CHLAMYDIA-TRACHOMATIS; CT/NG TEST; SYSTEM; URINE; AGE; DNA;
D O I：
10.1016/j.diagmicrobio.2015.09.020
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
PCR assays are nowadays between the most sensitive and reliable methods for screening and diagnosing sexually transmitted infections (STIs). The aim of this study was to analyze the reliability, accuracy, and usefulness of the new NG OligoGen kit in comparison with the cobas 4800 assay for the detection of Neisseria gonorrhoeae in clinical samples. A prospective study was designed for detection of N. gonorrhoeae including urine samples (n = 152), rectal (n = 80), endocervical (n = 67), pharyngeal (n = 41), and urethral swabs (n = 5) that were sent from a regional STI clinic in Seville, Spain. Samples were collected from 255 (73.9%) men and 90 women. Sensitivity, specificity, positive and negative predicative values, and kappa value for N. gonorrhoeae detection using the NC OligoGen kit were 99.6%, 100%, 100%, 99.1%, and 0.99, respectively. Statistical data obtained in this study confirm the usefulness and reliable results of this new assay. (C) 2016 Elsevier Inc. All rights reserved.
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页码：4 / 6
页数：3
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