Performance of the NG OligoGen kit for the diagnosis of Neisseria gonorrhoeae: comparison with cobas 4800 assay

被引：0
|
作者：
Parra-Sanchez, M. [1 ]
Garcia-Rey, S. [1 ]
Marcuello, A. [2 ]
Zakariya-Yousef, I. [1 ]
Bernal, S. [1 ]
Pueyo, I. [3 ]
Martin-Mazuelos, E. [1 ]
Palomares, J. C. [1 ]
机构：
[1] Valme Univ Hosp, Unit Infect Dis & Clin Microbiol, Seville, Spain
[2] Operon Inmuno & Mol Diagnost, Zaragoza, Spain
[3] Ctr Sexually Transmitted Infect Seville, Seville, Spain
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2016年 / 84卷 / 01期
关键词：
Sexually transmitted infections; Neisseria gonorrhoeae; Urine; Diagnostic test; CHLAMYDIA-TRACHOMATIS; CT/NG TEST; SYSTEM; URINE; AGE; DNA;
D O I：
10.1016/j.diagmicrobio.2015.09.020
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
PCR assays are nowadays between the most sensitive and reliable methods for screening and diagnosing sexually transmitted infections (STIs). The aim of this study was to analyze the reliability, accuracy, and usefulness of the new NG OligoGen kit in comparison with the cobas 4800 assay for the detection of Neisseria gonorrhoeae in clinical samples. A prospective study was designed for detection of N. gonorrhoeae including urine samples (n = 152), rectal (n = 80), endocervical (n = 67), pharyngeal (n = 41), and urethral swabs (n = 5) that were sent from a regional STI clinic in Seville, Spain. Samples were collected from 255 (73.9%) men and 90 women. Sensitivity, specificity, positive and negative predicative values, and kappa value for N. gonorrhoeae detection using the NC OligoGen kit were 99.6%, 100%, 100%, 99.1%, and 0.99, respectively. Statistical data obtained in this study confirm the usefulness and reliable results of this new assay. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：4 / 6
页数：3
相关论文
共 45 条
  • [1] Comparison of the CT OligoGen kit with cobas 4800 assay for detection of Chlamydia trachomatis
    Parra-Sanchez, Manuel
    Marcuello-Lopez, Ana
    Garcia-Rey, Silvia
    Zakariya-Yousef, Ismail
    Sivianes-Valdecantos, Nieves
    Sierra-Atienza, Celestina
    Bernal-Martinez, Samuel
    Pueyo-Rodrigez, Isabel
    Martin-Mazuelos, Estrella
    Carlos Palomares-Folia, Jose
    ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, 2015, 33 (10): : 642 - 645
  • [2] Is confirmatory testing of Roche cobas 4800 CT/NG test Neisseria gonorrhoeae positive samples required? Comparison of the Roche cobas 4800 CT/NG test with an opa/pap duplex assay for the detection of N gonorrhoeae
    Perry, Michael D.
    Jones, Rachel N.
    Corden, Sally A.
    SEXUALLY TRANSMITTED INFECTIONS, 2014, 90 (04) : 303 - 308
  • [3] Cobas® 4800: a fully automated system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae
    Van der Pol, Barbara
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2013, 13 (02) : 131 - 140
  • [4] Despite Excellent Test Characteristics of the cobas 4800 CT/NG Assay, Detection of Oropharyngeal Chlamydia trachomatis and Neisseria gonorrhoeae Remains Challenging
    van Niekerk, J. M.
    van der Veer, B. M. J. W.
    Hoebe, C. J. P. A.
    van de Bovenkamp, J.
    van Herk, C.
    van Loo, I. H. M.
    van Alphen, L. B.
    Wolffs, P. F. G.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2021, 59 (02)
  • [5] Evaluation of the cobas 4800 CT/NG Test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae DNA in urogenital swabs and urine specimens
    Parra-Sanchez, Manuel
    Carlos Palomares, Jose
    Bernal, Samuel
    Trinidad Gonzalez, Maria
    Sivianes, Nieves
    Perez, Luis
    Pueyo, Isabel
    Martin-Mazuelos, Estrella
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 74 (04) : 338 - 342
  • [6] A profile of the cobas® CT/NG assay on the cobas® 6800/8800 system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae
    Van Der Pol, Barbara
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2020, 20 (04) : 375 - 380
  • [7] Detection of Neisseria gonorrhoeae or Chlamydia trachomatis from oral wash specimens using the Abbott RealTime CT/NG assay and the Cobas 4800 CT/NG assay: A prospective study
    Matsumoto, Masahiro
    Hamasuna, Ryoichi
    Le, Phuong Thi
    Fujimoto, Naohiro
    Matsumoto, Tetsuro
    JOURNAL OF INFECTION AND CHEMOTHERAPY, 2022, 28 (04) : 480 - 485
  • [8] Comparison of GMT presto assay and Roche cobase® 4800 CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in dry swabs
    de Waaij, Dewi J.
    Dubbink, Jan Henk
    Peters, Remco P. H.
    Ouburg, Sander
    Morre, Servaas A.
    JOURNAL OF MICROBIOLOGICAL METHODS, 2015, 118 : 70 - 74
  • [9] Vaginal Swabs Are the Optimal Specimen for Detection of Genital Chlamydia trachomatis or Neisseria gonorrhoeae Using the Cobas 4800 CT/NG Test
    Van Der Pol, Barbara
    Taylor, Stephanie N.
    Liesenfeld, Oliver
    Williams, James A.
    Hook, Edward W., III
    SEXUALLY TRANSMITTED DISEASES, 2013, 40 (03) : 247 - 250
  • [10] Evaluation of the Abbott RealTime CT/NG Assay in Comparison to the Roche Cobas Amplicor CT/NG Assay
    Cheng, Annie
    Qian, Qinfang
    Kirby, James E.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) : 1294 - 1300
12345