Chemical Composition and Biological Activity of Allium cepa L. and Allium x cornutum (Clementi ex Visiani 1842) Methanolic Extracts

被引：77

作者：

Fredotovic, Zeljana ^{[1
]}

Sprung, Matilda ^{[2
]}

Soldo, Barbara ^{[2
]}

Ljubenkov, Ivica ^{[2
]}

Budic-Leto, Irena ^{[3
]}

Bilusic, Tea ^{[4
]}

Cikes-Culic, Vedrana ^{[5
]}

Puizina, Jasna ^{[1
]}

机构：

[1] Univ Split, Dept Biol, Fac Sci, R Boskovica 33, Split 21000, Croatia

[2] Univ Split, Dept Chem, Fac Sci, R Boskovica 33, Split 21000, Croatia

[3] Inst Adriat Crops & Karst Reclamat, Put Duilova 11, Split 21000, Croatia

[4] Univ Split, Fac Chem & Technol, Dept Food Technol & Biotechnol, R Boskovica 35, Split 21000, Croatia

[5] Univ Split, Sch Med, Dept Med Chem & Biochem, Soltanska 2, Split 21000, Croatia

来源：

MOLECULES | 2017年 / 22卷 / 03期

关键词：

onions; Allium x cornutum; Allium cepa; phenolic compounds; antioxidant activity; genotoxicity; antimutagenic activity; antiproliferative activity; PERFORMANCE LIQUID-CHROMATOGRAPHY; ANTIOXIDANT ACTIVITY; DNA-DAMAGE; HUMAN-LYMPHOCYTES; OXIDATIVE DAMAGE; ONION; QUERCETIN; VARIETIES; WILD; GLUCOSIDES;

D O I：

10.3390/molecules22030448

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we report a comparative study of the phytochemical profile and the biological activity of two onion extracts, namely Allium cepa L. and Allium x cornutum (Clementi ex Visiani 1842), members of the family Amaryllidaceae. The identification of flavonoids and anthocyanins, and their individual quantities, was determined by high-performance liquid chromatography (HPLC). The potency of both extracts to scavenge free radicals was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging activity and oxygen radical absorbance capacity (ORAC) methods. The DNA protective role was further tested by the single-cell gel electrophoresis (COMET) assay and by Fenton's reagent causing double-strand breaks on the closed circular high copy pUC19 plasmid isolated from Escherichia coli. In the presence of both extracts, a significant decrease in DNA damage was observed, which indicates a protective role of Allium cepa and Allium x cornutum on DNA strand breaks. Additionally, cytotoxicity was tested on glioblastoma and breast cancer cell lines. The results showed that both extracts had antiproliferative effects, but the most prominent decrease in cellular growth was observed in glioblastoma cells.

引用

页数：13

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