Sterol content analysis suggests altered eburicol 14α-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides

被引：24

作者：

Bean, Tim P. ^{[1
]}

Cools, Hans J. ^{[1
]}

Lucas, John A. ^{[1
]}

Hawkins, Nathaniel D. ^{[2
]}

Ward, Jane L. ^{[2
]}

Shaw, Michael W. ^{[3
]}

Fraaije, Bart A. ^{[1
]}

机构：

[1] Ctr Sustainable Pest & Dis Management, Dept Plant Pathol & Microbiol, Harpenden AL5 2JQ, Herts, England

[2] Natl Ctr Plant & Microbial Metabol, Harpenden, Herts, England

[3] Univ Reading, Sch Biol Sci, Reading, Berks, England

来源：

FEMS MICROBIOLOGY LETTERS | 2009年 / 296卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Septoria tritici; azole fungicide; eburicol; 14; alpha-demethylase; CYP51; sterol content; RESISTANT CANDIDA-ALBICANS; ANTIFUNGAL AGENTS; FLUCONAZOLE; MECHANISMS; AMPHOTERICIN; MUTATIONS; STRAINS;

D O I：

10.1111/j.1574-6968.2009.01645.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The recent decline in the effectiveness of some azole fungicides in controlling the wheat pathogen Mycosphaerella graminicola has been associated with mutations in the CYP51 gene encoding the azole target, the eburicol 14 alpha-demethylase (CYP51), an essential enzyme of the ergosterol biosynthesis pathway. In this study, analysis of the sterol content of M. graminicola isolates carrying different variants of the CYP51 gene has revealed quantitative differences in sterol intermediates, particularly the CYP51 substrate eburicol. Together with CYP51 gene expression studies, these data suggest that mutations in the CYP51 gene impact on the activity of the CYP51 protein.

引用

页码：266 / 273

页数：8

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