Detection and release of allergenic proteins in Parietaria judaica pollen grains

被引:8
作者
Vega-Maray, A. M.
Fernandez-Gonzalez, D.
Valencia-Barrera, R.
Suarez-Cervera, M.
机构
[1] Univ Leon, Fac Biol, Dept Plant Biol, E-24071 Leon, Spain
[2] Univ Barcelona, Fac Pharm, Dept Bot, Barcelona, Spain
关键词
Parietaria judaica; pollen; allergenic protein; localization; diffusion;
D O I
10.1007/s00709-006-0172-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rapid diffusion of allergenic proteins in isotonic media has been demonstrated for different pollen grains. Upon contact with stigmatic secretion or with the mucosa of sensitive individuals, pollen grains absorb water and release soluble low-molecular-weight proteins, these proteins enter in the secretory pathway in order to arrive at the cell surface. In this study we located allergenic proteins in mature and hydrated-activated pollen grains of Parietaria judaica L. (Urticaceae) and studied the diffusion of these proteins during the first 20 min of the hydration and activation processes. A combination of transmission electron microscopy and immunocytochemical methods was used to locate these proteins in mature pollen and in pollen grains after different periods of hydration and activation processes. Activated proteins reacting with antibodies in human serum from allergic patients were found in the cytoplasm, wall, and exudates from the pollen grains. The allergenic component of these pollen grains changes according to the pollen state; the presence of these proteins in the exine, the cytoplasm, and especially in the intine and in the material exuded from the pollen grains, is significant in the hydrated-activated studied times, whereas this presence is not significant in mature pollen grains. The rapid activation and release of allergenic proteins of P. judaica pollen appears to be the main cause of the allergenic activity of these pollen grains.
引用
收藏
页码:115 / 120
页数:6
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