Molecular detection of Rickettsia conorii and other zoonotic spotted fever group rickettsiae in ticks, Romania

被引:27
作者
Ionita, Mariana [1 ]
Silaghi, Cornelia [2 ]
Mitrea, Ioan Liviu [1 ]
Edouard, Sophie [3 ]
Parola, Philippe [3 ]
Pfister, Kurt [2 ]
机构
[1] Univ Agron Sci & Vet Med Bucharest, Fac Vet Med, Dept Parasitol & Parasit Dis, Bucharest 050097, Romania
[2] Univ Munich, Comparat Trop Med & Parasitol, D-81377 Munich, Germany
[3] Aix Marseille Univ, URMITE, WHO Collaborat Ctr Rickettsioses & Other Arthropo, UM63,CNRS 7278,IRD 198,Inserm 1095,Fac Med, Marseille, France
关键词
Spotted fever group rickettsiae; Ehrlichia canis; Babesia canis; Ticks; Dog; Romania; BORNE RICKETTSIOSES; EHRLICHIA-CANIS; DOGS; MONACENSIS; PATHOGENS; DISEASES; WORLD;
D O I
10.1016/j.ttbdis.2015.10.006
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The diverse tick fauna as well as the abundance of tick populations in Romania represent potential risks for both human and animal health. Spotted fever group (SFG) rickettsiae are recognized as important agents of emerging human tick-borne diseases worldwide. However, the epidemiology of rickettsial diseases has been poorly investigated in Romania. In urban habitats, companion animals which are frequently exposed to tick infestation, play a role in maintenance of tick populations and as reservoirs of tick-borne pathogens. Therefore, the aim of the present study was to investigate the occurrence of SFG rickettsiae in ticks infesting dogs in a greater urban area in South-eastern Romania. Adult ixodid ticks (n = 205), including Rhipicephalus sanguineus sensu lato (n=120), Dermacentor reticulatus (n = 76) and Ixodes ricinus (n=9) were collected from naturally infested dogs and were screened for SFG rickettsiae using conventional PCR followed by sequencing. Additionally, ticks were screened for DNA of Babesia spp., Hepatozoon spp., Ehrlichia canis, and Anaplasma platys. Four zoonotic SFG rickettsiae were identified: Rickettsia raoultii (16%) and Rickettsia slovaca (3%) in D. reticulatus, Rickettsia monacensis (11%) in I. ricinus, and Rickettsia conorii (0.8%) in Rh. sanguineus s.l. Moreover, pathogens of veterinary importance, such as B. canis (21%) in D. reticulatus and E. canis (7.5%) in Rh. sanguineus s.l. were identified. The findings expand the knowledge on distribution of SFG rickettsiae as well as canine pathogens in Romania. Additionally, this is the first report describing the molecular detection of R. conorii in ticks from Romania.. (C) 2015 Elsevier GmbH. All rights reserved.
引用
收藏
页码:150 / 153
页数:4
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