Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)

被引：17

作者：

Li, L. ^{[1
]}

Li, N. ^{[1
]}

Song, S. F. ^{[1
]}

Li, Y. X. ^{[3
]}

Xia, X. J. ^{[2
]}

Fu, X. Q. ^{[1
]}

Chen, G. H. ^{[3
]}

Deng, H. F. ^{[1
]}

机构：

[1] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China

[2] Chinese Acad Sci, Inst Subtrop Agr, Changsha, Hunan, Peoples R China

[3] Hunan Agr Univ, Coll Agr, Changsha, Hunan, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2014年 / 13卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Rice (Oryza sativa L.); Drought resistance; Drought-resistance mechanisms; OsRCI2-5; AGROBACTERIUM-MEDIATED TRANSFORMATION; TRANSCRIPTION FACTORS; COLD-ACCLIMATION; WATER-STRESS; SALT STRESS; EXPRESSION; TOLERANCE; PROTEIN; DEHYDRATION; SENSITIVITY;

D O I：

10.4238/2014.May.23.13

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei'ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated and downregulated genes. In this study, we screened the drought-resistant gene OsRCI2-5, after which a constitutive OsRCI2-5 construct was created and transferred into Nipponbare. After polyethylene glycol-6000 and drought treatment, we found that the OsRCI2-5 gene improved the drought resistance of Nipponbare. Gene expression profiling showed that the OsRCI2-5 gene was expressed in the rice leaves, stems, and flower organs. Subcellular localization revealed that the gene was located in the membranes, and hence, we can deduce that a membrane signal peptide was responsible for signal transduction.

引用

页码：4022 / 4035

页数：14

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