Altered expression of selected microRNAs in melanoma: Antiproliferative and proapoptotic activity of miRNA-155

被引：78

作者：

Levati, Lauretta ^{[1
]}

Alvino, Ester ^{[2
]}

Pagani, Elena ^{[1
]}

Arcelli, Diego ^{[1
]}

Caporaso, Patrizia ^{[1
]}

Bondanza, Sergio ^{[3
]}

Di Leva, Gianpiero ^{[4
,5
]}

Ferracin, Manuela ^{[6
,7
]}

Volinia, Stefano ^{[4
,5
,8
]}

Bonmassar, Enzo ^{[2
,9
]}

Croce, Carlo Maria ^{[4
,5
]}

D'Atri, Stefania ^{[1
]}

机构：

[1] Ist Dermopat Immacolata, IRCCS, Mol Oncol Lab, I-00167 Rome, Italy

[2] CNR, Inst Neurobiol & Mol Med, Dept Med, I-00133 Rome, Italy

[3] Ist Dermopat Immacolata, IRCCS, Lab Tissue Engn & Cutaneous Physiopathol, I-00167 Rome, Italy

[4] Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA

[5] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA

[6] Univ Ferrara, Dept Expt & Diagnost Med, I-44100 Ferrara, Italy

[7] Univ Ferrara, Interdept Ctr Canc Res, I-44100 Ferrara, Italy

[8] Univ Ferrara, DAMA, Dept Morphol & Embryol, I-44100 Ferrara, Italy

[9] Univ Roma Tor Vergata, Dept Neurosci, Sch Med, I-00133 Rome, Italy

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2009年 / 35卷 / 02期

关键词：

microRNA; microRNA-155; melanoma; proliferation; apoptosis; CANCER CELL-PROLIFERATION; MALIGNANT-MELANOMA; SOLID TUMORS; KAPPA-B; TARGETS; PROGRESSION; GROWTH; IDENTIFICATION; MECHANISMS; SIGNATURES;

D O I：

10.3892/ijo_00000352

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Altered expression of microRNAs (miRNAs) has been detected in cancer, suggesting that these small non-coding RNAs can act as oncogenes or tumor suppressor genes. In the present study, we investigated the expression of miRNA-17-5p, miRNA-18a, miRNA-20a, miRNA-92a, miRNA-146a, miRNA-146b and miRNA-155 by real-time quantitative RT-PCR in a panel of melanocyte cultures and melanoma cell lines and explored the possible role of miRNA-155 in melanoma cell proliferation and survival. The analyzed miRNAs were selected on the basis of previous Studies strongly supporting their involvement in cancer development and/or progression. We found that miRNA-17-5p, miRNA-18a, miRNA-20a, and miRNA-92a were overexpressed, whereas miRNA-146a, miRNA-146b and miRNA-155 were down-regulated in the majority of melanoma cell lines with respect to melanocytes. Ectopic expression of miRNA-155 significantly inhibited proliferation in 12 of 13 melanoma cell lines with reduced levels of this miRNA and induced apoptosis in 4 out of 4 cell lines analyzed. In conclusion, our data further support the finding of altered miRNA expression in melanoma cells and establish for the first time that miRNA-155 is a negative regulator of melanoma cell proliferation and survival.

引用

页码：393 / 400

页数：8

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