Angiotensin II stimulates endothelial vascular cell adhesion molecule-1 via nuclear factor-κB activation induced by intracellular oxidative stress

被引:442
|
作者
Pueyo, ME
Gonzalez, W
Nicoletti, A
Savoie, F
Arnal, JF
Michel, JB
机构
[1] Univ Paris 07, INSERM U460, F-75018 Paris, France
[2] INSERM U430, Paris, France
[3] INSERM U397, Toulouse, France
关键词
endothelium; nuclear factor-kappa B; I kappa B; superoxide anions; hydrogen peroxide;
D O I
10.1161/01.ATV.20.3.645
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The recruitment of monocytes via the endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) is a key step in the formation of the initial lesion in atherosclerosis. Because angiotensin (Ang) II may be involved in this process, we investigated its role on the signaling cascade leading to VCAM-1 expression in endothelial cells. Ang II stimulates mRNA and protein expression of VCAM-1 in these cells via the AT(1) receptor. This effect was enhanced by N-G-nitro-L-arginine methyl ester, a nitric oxide synthase inhibitor, and blocked by pyrrolidinedithiocarbamate, an antioxidant molecule. Ang II activated the redox-sensitive transcription factor nuclear factor-KB and stimulated the degradation of both inhibitor of kappa B (I kappa B)alpha and I kappa B beta with different kinetics. The degradation of I kappa Bs induced by Ang II was not modified by incubation with exogenous superoxide dismutase and catalase, suggesting that this effect was not mediated by the extracellular production of O-2(-). In contrast, rotenone and antimycin, 2 inhibitors of the mitochondrial respiratory chain, inhibited the Ang II-induced I kappa B degradation, showing that generation of reactive oxygen species in the mitochondria is involved on Ang II action. BXT-51702, a glutathione peroxidase mimic, inhibited the effect of Ang II, and aminotriazole, an inhibitor of catalase, enhanced it, suggesting a role for H2O2 in I kappa B degradation. This is confirmed by experiments showing that Ang II stimulates the intracellular production of H2O2 in endothelial cells. These results demonstrate that Ang II induced an intracellular oxidative stress in endothelial cells, which stimulates I kappa B degradation and nuclear factor-kappa B activation. This activation enhances the expression of VCAM-1 and probably other genes involved in the early stages of atherosclerosis.
引用
收藏
页码:645 / 651
页数:7
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