Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats

被引:24
作者
Qiao, Yu-Feng [1 ,2 ]
Guo, Wen-Juan [1 ]
Li, Lu [3 ]
Shao, Shan [1 ]
Qiao, Xi [1 ]
Shao, Jin-Jin [1 ]
Zhang, Qiong [4 ]
Li, Rong-Shan [4 ]
Wang, Li-Hua [1 ]
机构
[1] Shanxi Med Univ, Hosp 2, Dept Nephrol, Taiyuan 030001, Shanxi, Peoples R China
[2] Shanxi Med Univ, Clin Coll 2, Sch Med, Taiyuan 030001, Shanxi, Peoples R China
[3] Baoding 1 Hosp, Dept Nephrol, Baoding 071000, Hebei, Peoples R China
[4] Shanxi Peoples Hosp, Dept Nephrol, Taiyuan 030001, Shanxi, Peoples R China
关键词
melatonin; hypertension; oxidative stress; renal injury; FREE RADICAL ACTIVITY; REACTIVE OXYGEN; DISEASES; PROTEIN; KIDNEY; WOMEN; RISK; MICE;
D O I
10.3892/mmr.2015.4495
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The aim of the present study was to investigate the protective effects of melatonin (MLT) on hypertension-induced renal injury and identify its mechanism of action. Twenty-four healthy male Wistar rats were divided into a sham control group (n=8), which was subjected to sham operation and received vehicle treatment (physiological saline intraperitoneally at 0.1 ml/100 g), a vehicle group (n=8), which was subjected to occlusion of the left renal artery and vehicle treatment, and the MLT group (n=8), which was subjected to occlusion of the left renal artery and treated with MLT (10 mg/kg/day). Pathological features of the renal tissues were determined using hematoxylin and eosin staining and Masson staining. Urine protein, serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined. Immunohistochemical analysis was performed to determine the expression of heme oxygenase-1 (HO-1), intercellular adhesion molecule-1 (ICAM-1), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS). Furthermore, reverse transcription polymerase chain reaction was conducted to determine the mRNA expression of HO-1, ICAM-1, eNOS and iNOS. A marked decrease in blood pressure was noticed in the MLT group at week 4 compared with that of the vehicle group (P<0.01). Furthermore, MLT treatment attenuated the infiltration of inflammatory cells and oedema/atrophy of renal tubules. MLT attenuated hypertension-induced increases in urine protein excretion, serum creatinine and MDA as well as decreases in SOD activity in renal tissues. Furthermore, MLT attenuated hypertension-induced increases in iNOS and ICAM-1 as well as decreases in eNOS and HO-1 expression at the mRNA and protein level. In conclusion, the results of the present study indicated that MLT had protective roles in hypertension-induced renal injury. Its mechanism of action is, at least in part, associated with the inhibition of oxidative stress.
引用
收藏
页码:21 / 26
页数:6
相关论文
共 50 条
[41]   Melatonin attenuates aortic oxidative stress injury and apoptosis in STZ-diabetes rats by Notch1/Hes1 pathway [J].
Ren, Bin-Cheng ;
Zhang, Wen ;
Zhang, Wei ;
Ma, Jian-Xing ;
Pei, Fei ;
Li, Bu-Ying .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2021, 212
[42]   Celastrol Attenuates Hypertension-Induced Inflammation and Oxidative Stress in Vascular Smooth Muscle Cells via Induction of Heme Oxygenase-1 [J].
Yu, Xiao ;
Tao, Weiwei ;
Jiang, Fengrong ;
Li, Chaojun ;
Lin, Jiandie ;
Liu, Chang .
AMERICAN JOURNAL OF HYPERTENSION, 2010, 23 (08) :895-903
[43]   Melatonin reduces traumatic brain injury-induced oxidative stress in the cerebral cortex and blood of rats [J].
Senol, Nilgun ;
Naziroglu, Mustafa .
NEURAL REGENERATION RESEARCH, 2014, 9 (11) :1112-1116
[44]   Strength Training Reduces Cardiac and Renal Oxidative Stress in Rats with Renovascular Hypertension [J].
Miguel-dos-Santos, Rodrigo ;
dos Santos, Jucilene Freitas ;
Macedo, Fabricio Nunes ;
Marcal, Anderson Carlos ;
Santana-Filho, Valter J. ;
Wichi, Rogerio Brandao ;
Lauton-Santos, Sandra .
ARQUIVOS BRASILEIROS DE CARDIOLOGIA, 2021, 116 (01) :4-11
[45]   Melatonin reduces traumatic brain injury-induced oxidative stress in the cerebral cortex and blood of rats [J].
Nilgn Senol ;
Mustafa Naziroglu .
NeuralRegenerationResearch, 2014, 9 (11) :1112-1116
[46]   Bilirubin attenuates the renal tubular injury by inhibition of oxidative stress and apoptosis [J].
Se Won Oh ;
Eun Seong Lee ;
Sejoong Kim ;
Ki Young Na ;
Dong Wan Chae ;
Suhnggwon Kim ;
Ho Jun Chin .
BMC Nephrology, 14
[47]   Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats [J].
Aydemir, Sezgin ;
Akgun, Sevcan Gul ;
Beceren, Ayfer ;
Yuksel, Meral ;
Kumas, Meltem ;
Erdogan, Nusret ;
Sardas, Semra ;
Omurtag, Gulden Zehra .
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2017, 10 (04) :6250-6261
[48]   Dihydroartemisinin attenuates lipopolysaccharide-induced acute kidney injury by inhibiting inflammation and oxidative stress [J].
Liu, Xinhui ;
Lu, Jiandong ;
Liao, Yijiao ;
Liu, Siqi ;
Chen, Yijun ;
He, Riming ;
Men, Ling ;
Lu, Chunjian ;
Chen, Zhihong ;
Li, Shunmin ;
Xiong, Guoliang ;
Yang, Shudong .
BIOMEDICINE & PHARMACOTHERAPY, 2019, 117
[49]   Melatonin prevents methotrexate-induced hepatorenal oxidative injury in rats [J].
Jahovic, N ;
Çevik, H ;
Sehirli, AÖ ;
Yegen, BÇ ;
Sener, G .
JOURNAL OF PINEAL RESEARCH, 2003, 34 (04) :282-287
[50]   Ferulic acid alleviates avermectin induced renal injury in carp by inhibiting inflammation, oxidative stress and apoptosis [J].
Li, Qiulu ;
Ping, Kaixin ;
Xiang, Yannan ;
Sun, Ying ;
Hu, Zunhan ;
Liu, Shujuan ;
Guan, Xinying ;
Fu, Mian .
FISH & SHELLFISH IMMUNOLOGY, 2024, 149