Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides

被引：191
作者：
Andreescu, S
Barthelmebs, L
Marty, JL
机构：
[1] Univ Perpignan, Ctr Phytopharm, URA 5054, F-66860 Perpignan, France
[2] Univ Bucharest, Fac Chem, Dept Analyt Chem, Bucharest 76234, Romania
来源：
ANALYTICA CHIMICA ACTA | 2002年 / 464卷 / 02期
关键词：
biosensor; acetylcholinesterase; immobilization; screen-printed; pesticides;
D O I：
10.1016/S0003-2670(02)00518-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The analytical performance of three acetylcholinesterase (AChE) screen-printed biosensors, designed for the detection of pesticides are evaluated. Bioencapsulation of the enzyme in a sol-gel composite and immobilization by metal-chelate affinity were compared with the entrapment of the enzyme in a photopolymerisable polymer. A very low amount of enzyme ranging between 0.8 and 1.2 mIU was immobilized on the electrode surface in each approach. The sensors exhibited a storage stability of over 6 months when the enzyme was encapsulated in a polymer film. Pesticide concentrations in the range of 10(-8) to 10(-9) M paraoxon, dichlorvos and chlorpyrifos ethyl oxon could be detected according to each configuration by following an incubation time of 20 min. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：171 / 180
页数：10
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